Browse > Article
http://dx.doi.org/10.7314/APJCP.2013.14.12.7315

Overexpressed Ostepontin-c as a Potential Biomarker for Esophageal Squamous Cell Carcinoma  

Zhang, Mei-Xiang (Department of Clinical Lab, Nanjing Medical University Cancer Hospital & Jiangsu Cancer Hospital)
Xu, Yi-Jun (Department of Gastroenterology, Nanjing First Hospital & Nanjing Medical University First Hospital)
Zhu, Ming-Chen (Department of Clinical Lab, Nanjing Medical University Cancer Hospital & Jiangsu Cancer Hospital)
Yan, Feng (Department of Clinical Lab, Nanjing Medical University Cancer Hospital & Jiangsu Cancer Hospital)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.12, 2013 , pp. 7315-7319 More about this Journal
Abstract
Background: The metastasis gene osteopontin (OPN) is subject to alternative splicing, which yields three messages, osteopontin-a, osteopontin-b and osteopontin-c. Osteopontin-c is selectively expressed in invasive, but not in noninvasive tumors. In the present study, we examined the expression of OPN-c in esophageal squamous cell carcinomas (ESCCs) and assessed its value as a diagnostic biomarker. Methods: OPN-c expression was assessed by immunohistochemistry in 63 ESCC samples and correlated with clinicopathologic factors. Expression was also examined in peripheral blood mononuclear cells (PBMCs) from 120 ESCC patients and 30 healthy subjects. The role of OPN-c mRNA as a tumor marker was investigated by receiver operating characteristic curve (ROC) analysis. Results: Immunohistochemistry showed that OPN-c was expressed in 30 of 63 cancer lesions (48%)and significantly associated with pathological T stage (P=0.038) and overall stage (P=0.023). Real time PCR showed that OPN-c mRNA was expressed at higher levels in the PBMCs of ESCC patients than in those of healthy subjects (P<0.0001) with a sensitivity as an ESCC biomarker of 86.7%. Conclusion: Our findings suggest that expression of OPN-c is significantly elevated in ESCCs and this upregulation could be a potential diagnostic marker.
Keywords
OPN-c; esophageal squamous cell carcinoma; biomarker; immunohistochemistry;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Lin JY, Wang MS, Dong LP, et al (2012). Influence of personal character on quality of life of patients with esophageal cancer in north Henan province and influencing factors. Asian Pac J Cancer Prev, 13, 5415-20.   과학기술학회마을   DOI   ScienceOn
2 Livak KJ, Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative pcr and the 2 (-delta delta c (t)) method. Methods, 25, 402-8.   DOI   ScienceOn
3 Ma JB, Chen EC, Song YP, et al (2013). Prognostic significance of 18F-fluorodeoxyglucose positron emission tomography (PET)-based parameters in neoadjuvant chemoradiation treatment of esophageal carcinoma. Asian Pac J Cancer Prev, 14, 2477-81.   과학기술학회마을   DOI   ScienceOn
4 Mirza M, Shaughnessy E, Hurley JK, et al (2008). Osteopontin-c is a selective marker of breast cancer. Int J Cancer, 122, 889-97.   DOI   ScienceOn
5 Schorge JO, Drake RD, Lee H, et al (2004). Osteopontin as an adjunct to ca125 in detecting recurrent ovarian cancer. Clin Cancer Res, 10, 3474-8.   DOI   ScienceOn
6 Senger DR, Ledbetter SR, Claffey KP, et al (1996). Stimulation of endothelial cell migration by vascular permeability factor/vascular endothelial growth factor through cooperative mechanisms involving the alphavbeta3 integrin, osteopontin, and thrombin. Am J Pathol, 149, 293-305.
7 Sodek J, Ganss B, McKee MD (2000). Osteopontin. Crit Rev Oral Biol Med, 11, 279-303.   DOI   ScienceOn
8 Thalmann GN, Sikes RA, Devoll RE, et al (1999). Osteopontin: Possible role in prostate cancer progression. Clin Cancer Res, 5, 2271-7.
9 Tuck AB, Chambers AF (2001). The role of osteopontin in breast cancer: Clinical and experimental studies. J Mammary Gland Biol Neoplasia, 6, 419-29.   DOI   ScienceOn
10 Vizcaino AP, Moreno V, Lambert R, et al (2002). Time trends incidence of both major histologic types of esophageal carcinomas in selected countries. Int J Cancer, 99, 860-8.   DOI   ScienceOn
11 Wang S, Liu H, Akhtar J, et al (2013). Alteration of runt-related transcription factor 3 gene expression and biologic behavior of esophageal carcinoma TE-1 cells after 5-azacytidine intervention. Asian Pac J Cancer Prev, 14, 5427-33.   과학기술학회마을   DOI   ScienceOn
12 Weber GF, Ashkar S, Glimcher MJ, et al (1996). Receptor-ligand interaction between cd44 and osteopontin (eta-1). Science, 271, 509-12.   DOI   ScienceOn
13 Ye QH, Qin LX, Forgues M, et al (2003). Predicting hepatitis b virus-positive metastatic hepatocellular carcinomas using gene expression profiling and supervised machine learning. Nat Med, 9, 416-23.   DOI   ScienceOn
14 He B, Mirza M, Weber GF (2006). An osteopontin splice variant induces anchorage independence in human breast cancer cells. Oncogene, 25, 2192-202.   DOI   ScienceOn
15 Agrawal D, Chen T, Irby R, et al (2002). Osteopontin identified as lead marker of colon cancer progression, using pooled sample expression profiling. J Natl Cancer Ins, 94, 513-21.   DOI   ScienceOn
16 Barry ST, Ludbrook SB, Murrison E, et al (2000). Analysis of the alpha4beta1 integrin-osteopontin interaction. Exp Cell Res, 258, 342-51.   DOI   ScienceOn
17 Chambers AF, Wilson SM, Kerkvliet N,et al (1996). Osteopontin expression in lung cancer. Lung Cancer, 15, 311-23.   DOI   ScienceOn
18 Furger KA, Allan AL, Wilson SM, et al (2003). Beta (3) integrin expression increases breast carcinoma cell responsiveness to the malignancy-enhancing effects of osteopontin. Mol Cancer Res, 1, 810-9.
19 Gardner HA, Berse B, Senger DR (1994). Specific reduction in osteopontin synthesis by antisense rna inhibits the tumorigenicity of transformed rat1 fibroblasts. Oncogene, 9, 2321-6.
20 Hofstetter W, Swisher SG, Correa AM, et al (2002). Treatment outcomes of resected esophageal cancer. Ann Surg, 236, 376-84; discussion 84-5.   DOI   ScienceOn
21 Ito T, Hashimoto Y, Tanaka E, et al (2006). An inducible shorthairpin rna vector against osteopontin reduces metastatic potential of human esophageal squamous cell carcinoma in vitro and in vivo. Clin Cancer Res, 12, 1308-16.   DOI   ScienceOn
22 Katagiri YU, Sleeman J, Fujii H, et al (1999). Cd44 variants but not cd44s cooperate with beta1-containing integrins to permit cells to bind to osteopontin independently of arginineglycine-aspartic acid, thereby stimulating cell motility and chemotaxis. Cancer Res, 59, 219-26.
23 Kita Y, Natsugoe S, Okumura H, et al (2006). Expression of osteopontin in oesophageal squamous cell carcinoma. Br J Cancer, 95, 634-8.   DOI   ScienceOn
24 Lee JM, Wu MT, Lee YC, et al (2005). Association of gstp1 polymorphism and survival for esophageal cancer. Clin Cancer Res, 11, 4749-53.   DOI   ScienceOn