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http://dx.doi.org/10.7314/APJCP.2013.14.11.6945

Diosgenin Inhibits hTERT Gene Expression in the A549 Lung Cancer Cell Line  

Mohammad, Rahmati Yamchi (Drug Applied Research Center, Tabriz University of Medical Sciences)
Somayyeh, Ghareghomi (Department of Biology, Faculty of Sciences, Golestan University)
Gholamreza, Haddadchi (Department of Biology, Faculty of Sciences, Golestan University)
Majid, Mobasseri (Bone Health Research Center, Tabriz University of Medical Sciences)
Yousef, Rasmi (Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.11, 2013 , pp. 6945-6948 More about this Journal
Abstract
Background: Diosgenin, a steroidal saponin from a therapeutic herb, fenugreek (Trigonellafoenum-graceum L.), has been recognized to have anticancer properties. Telomerase activity is not detected in typical healthy cells, while in cancer cell telomerase expression is reactivated, therefore providing a promising cancer therapeutic target. Materials and Methods: We studied the inhibitory effect of diosgenin on human telomerase reverse transcriptase gene (hTERT) expression which is critical for telomerase activity. MTT- assays and qRT-PCR analysis were conducted to assess cytotoxicity and hTERT gene expression inhibition effects, respectively. Results: MTT results showed that $IC_{50}$ values for 24, 48 and 72h after treatment were 47, 44 and $43{\mu}M$, respectively. Culturing cells with diosgenin treatment caused down-regulation of hTERT expression. Discussion: These results show that diosgenin inhibits telomerase activity by down-regulation of hTERT gene expression in the A549 lung cancer cell line.
Keywords
Lung cancer; hTERT; diosgenin; telomerase; qRT-PCR; MTT assay;
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