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http://dx.doi.org/10.7314/APJCP.2013.14.11.6481

Induction of Cytotoxicity and Apoptosis in Human Gastric Cancer Cell SGC-7901 by Isovaltrate Acetoxyhydrin Isolated from Patrinia heterophylla Bunge Involves a Mitochondrial Pathway and G2/M Phase Cell Cycle Arrest  

Yang, Bo (College of Pharmaceutical Sciences, Zhejiang Chinese Medical University)
Wang, Yi-Qi (College of Pharmaceutical Sciences, Zhejiang Chinese Medical University)
Cheng, Ru-Bin (College of Pharmaceutical Sciences, Zhejiang Chinese Medical University)
Chen, Jia-Li (College of Pharmaceutical Sciences, Zhejiang Chinese Medical University)
Chen, Jin (Huadong Medicine Company Limited)
Jia, Li-Tao (Department of Gastroenterology, Tongde Hospital of Zhejiang Province)
Zhang, Ru-Song (College of Pharmaceutical Sciences, Zhejiang Chinese Medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.11, 2013 , pp. 6481-6486 More about this Journal
Abstract
Background: Our previous study demonstrated cytotoxicity of a crude extract from Patrinia heterophylla Bunge (PHEB). In the present study, we aimed to investigate the effects of isovaltrate acetoxyhydrin (IA) isolated from PHEB on the gastric cancer cell SGC-7901, in order to explore a potential treatment for gastric cancer. Methods: MTT assays were employed to determine the effects of IA on cell vitality and proliferation, with monitoring of cell morphology changes and examination of apoptosis with Annexin V-PI staining. Flow cytometry was used to assess cell cycle progression and mitochondrial membrane potential. The activity of caspase 3, 9 was evaluated by spectrophotometry, and the protein levels of Bax, Bcl2 and Cyclin B1 were analyzed with Western blotting of total proteins extracted from cultured cells. Results: The results demonstrated direct toxicity of IA towards SGC-7901 cells. Evidence of apoptosis included blebbing and chromatin condensation. Annexin V-PI assays revealed early apoptosis, involving rapid depolarization of mitochondrial membranes and activity of caspase 3, 9 signaling pathways. Western blotting showed that Bcl2 and Bax proteins was down- and up-regulated, respectively, and cyclin B1 was up-regulated. Cell cycle analysis further indicated that IA could induce G2/M phase arrest in SGC-7901 cells. Conclusions: In conclusion, we believe that IA induces apoptosis of SGC-7901 cells, therefore providing a potential therapeutic agent for treatment of gastric cancer.
Keywords
Patrinia heterophylla Bunge; isovaltrate acetoxyhydrin; SGC-7901; apoptosis; G2/M arrest;
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