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http://dx.doi.org/10.7314/APJCP.2013.14.11.6393

Kupffer Cells Suppress Hepatocarcinogenesis and Metastasis in Tumor Orthotopic Implanted Kunming Mice

Li, X.Y. (Department of Hepatobiliary surgery, Traditonal Chinese Medicine Hospital of Dianjiang)
Wang, M.Y. (Department of Hepatobiliary surgery, The Second Affiliated Hospital of Chongqing Medical University)
Zhang, J.Y. (Department of Hepatobiliary surgery, The Second Affiliated Hospital of Chongqing Medical University)
Li, J.Z. (Department of Hepatobiliary surgery, The Second Affiliated Hospital of Chongqing Medical University)
Gong, J.P. (Department of Hepatobiliary surgery, The Second Affiliated Hospital of Chongqing Medical University)
Zhang, Wei (Department of Hepatobiliary surgery, The Second Affiliated Hospital of Chongqing Medical University)

Publication Information

Asian Pacific Journal of Cancer Prevention / v.14, no.11, 2013 , pp. 6393-6398 More about this Journal

Abstract

In this research, we used $GdCl_3$ (gadolinium chloride) to restrain the function of Kupffer cells and assessed effects on hepatocarcinogenesis and metastasis in the Kunming mouse. A 0.25% $GdCl_3$ solution (10 mg/kg b.w.) was infused via the vena caudalis of each mouse 1 week before inoculation of H22 cells and was continued once per three days. Then we observed the follow indexes 3 weeks after injection of H22 cells: tumor weight, histologic characteristics of tumor tissue by light microscopy, ultramicrostructure of Kupffer cells under the electron microscope, distribution and number of Kupffer cells by histochemical staining, and TNF- ${\alpha}$ and IFN- ${\gamma}$ levels in blood-serum and liver tissue by ELISA and RT-PCR. MMP-2 protein expression was tested by immunohistochemistry. The $GdCl_3$ pretreatment had no effect on the quantity of Kupffer cells, but clearly restrained their functions, with decrease of TNF- ${\alpha}$ and IFN- ${\gamma}$ levels and elevation of MMP2. Tumor immunity functions were markedly suppressed and tumor growth was accelerated with appearance of metastasis. Furthermore, survival time of trial mice was shortened.

Keywords

Kupffer cells; hepatocellular carcinoma; gadolinium chloride; suppressive effects;

Citations & Related Records

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