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http://dx.doi.org/10.7314/APJCP.2012.13.8.3863

Mitochondrial D-Loop Polymorphism and Microsatellite Instability in Prostate Cancer and Benign Hyperplasia Patients  

Ashtiani, Zahra Ousati (Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences)
Heidari, Mansour (Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences)
Hasheminasab, Sayed-Mohammad (Department of Clinical Pharmacology, University Medical Center Gottingen (UMG))
Ayati, Mohsen (Department of Urology, Imam Khomeini General Hospital, Tehran University of Medical Sciences)
Rakhshani, Naser (Department of Pathology, Shariati Hospital, Tehran University of Medical Sciences)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.8, 2012 , pp. 3863-3868 More about this Journal
Abstract
In this study mitochondrial D-Loop variations in Iranian prostate cancer and benign prostatic hyperplasia (BPH) patients were investigated. Tumour samples and corresponding non-cancerous prostate tissue from 40 prostate cancer patients and 40 age-matched BPH patients were collected. The entire mtD-loop region (16024-576) was amplified using the PCR method and products were gel-purified and subjected to direct nucleotide sequencing. A total of 129 variations were found, the most frequent being 263A${\rightarrow}$G and 310T${\rightarrow}$C among both BPH and prostate cancer patients. Variation of 309 C${\rightarrow}$T was significantly more frequent in prostate cancer patients (P value<0.05). Four novel variations were observed on comparison with the MITOMAP database. Novel variations were np16154delT, np366G${\rightarrow}$A, np389G${\rightarrow}$A and 56insT. There was no correspondence between the different variations and the age of subjects. Considering that D-loop variations were frequent in both BPH and prostate cancer patients in our study, the fact that both groups had high average age can be a possible contributing factor. D-loop polymorphisms and microsatellite instability can influence cell physiology and result in a benign or malignant phenotype. Significantly higher frequency of 309 C${\rightarrow}$T variation in cancer patients is a notable finding and must be a focus of attention in future studies.
Keywords
Prostate cancer; mitochondrial DNA; D-Loop; benign prostatic hyperplasia (BPH);
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