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http://dx.doi.org/10.7314/APJCP.2012.13.8.3713

Transmembrane Protein 166 Expression in Esophageal Squamous Cell Carcinoma in Xinjiang, China  

Sun, Wei (Department of Thoracic Oncology, the Affiliated Tumor Hospital of Xinjiang Medical University)
Ma, Xiu-Min (State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, the First Affiliated Hospital of Xinjiang Medical University)
Bai, Jing-Ping (Department of Thoracic Oncology, the Affiliated Tumor Hospital of Xinjiang Medical University)
Zhang, Guo-Qing (Department of Thoracic Oncology, the Affiliated Tumor Hospital of Xinjiang Medical University)
Zhu, Yue-Jie (National Clinical Research Base of Traditional Chinese Medicine of Xinjiang Medical University)
Ma, Hai-Mei (State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, the First Affiliated Hospital of Xinjiang Medical University)
Guo, Hui (National Clinical Research Base of Traditional Chinese Medicine of Xinjiang Medical University)
Chen, Ying-Yu (Laboratory of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center)
Ding, Jian-Bing (National Clinical Research Base of Traditional Chinese Medicine of Xinjiang Medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.8, 2012 , pp. 3713-3716 More about this Journal
Abstract
Objective: Transmembrane protein 166 (TMEM166) expression in esophageal squamous cell carcinoma (ESCC) and remote normal esophageal tissues was examined to assess any role in tumour biology. Methods: TMEM166 mRNA expression in 36 cases with ESCC (36 tumour samples, 36 remote normal esophageal tissue samples) was detected by RT-PCR. TMEM166 protein expression was analysed in paraffin-embedded tissue samples from the same cases by immunohistochemistry. Results: Semi-quantitative analysis showed TMEM166 mRNA expression in ESCCs to be significantly lower than in remote normal esophageal tissues ($0.759{\pm}0.713$ vs. $2.622{\pm}1.690$, P=0.014). TMEM166 protein expression was also significantly reduced (69.4% vs. 94.4%, P<0.01). Conclusion: TMEM166 mRNA and protein expression demonstrated significant reduction in ESCCs compared with remote esophageal tissues, albeit with no correlation with tumour size, differentiation, stage, and lymph node metastasis, suggesting a role in regulating autophagic and apoptotic processes in the ESCC.
Keywords
Esophageal squamous cell carcinoma; transmembrane protein166; apoptosis; autophagy; RT-PCR;
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