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http://dx.doi.org/10.7314/APJCP.2012.13.7.3423

Rapid Detection of Ovarian Cancer from Immunized Serum Using a Quartz Crystal Microbalance Immunosensor  

Chen, Yan (School of Automation & Engineering, University of Electronic Science and Technology of China)
Huang, Xian-He (School of Automation & Engineering, University of Electronic Science and Technology of China)
Shi, Hua-Shan (State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Clinical Medicine School, Sichuan University)
Mu, Bo (North Sichuan Medical College)
Lv, Qun (Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.7, 2012 , pp. 3423-3426 More about this Journal
Abstract
Background: The objective of this study was to measure the antibody content of NuTu-19 ovarian cancer cells in serum samples using a quartz crystal microbalance (QCM) immunosensor. Materials and Methods: NuTu-19 cells were first cultured onto the electrode surfaces of crystals in Dulbecco's modified Eagle medium, and then specified amounts of immunized serum samples of immunized rabbit were also added. The change in mass caused by specific adsorbtion of antibodies of NuTu-19 to the surfaces of the crystals was detected. Results: The change in resonance frequency of crystals caused by immobilization of NuTu-19 cells was from 83 to 429Hz. The antibody content of NuTu-19 detected was 341ng/ul. The frequency shifts were linearly dependent on the amount of antibody mass in the range of 69 to 340ng. The positive detection rate and the negative detection rate were 80% and 100%, respectively. Conclusion: This immunoassay provides a viable alternative to other early ovarian cancer detection methods and is particularly suited for health screening of the general population.
Keywords
Ovariabn cancer; detection; quartz crystal microbalance; immunosensor; NuTu-19;
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  • Reference
1 Babacan S, Pivarnik P, Letcher S, et al (2009). Evaluation of antibody immobilization methods for piezoelectric biosensor application. Biosens Bioelectron, 15, 615-21.
2 Clarke-Pearson DL (2009). Screening for ovarian cancer. N Engl J Med, 361, 170-7.   DOI
3 Goff BA, Mandel L, Muntz HG, Melancon CH (2000). Ovarian carcinoma diagnosis. Cancer, 89, 2068-75.   DOI   ScienceOn
4 Granstaff VE, Martin SJ (1994). Characterization of a thickness-Shear mode quartz resonator with multiple nonpiezoelectric layers. J Appl Phys, 75, 1319-29.   DOI
5 Hlavay J, Gulbault GG (1977). Applications of the piezoelectric crystal detector in analytical chemistry. Anal Chem, 49, 1890-8.   DOI
6 Jelovac D, Armstrong DK (2011). Recent progress in the diagnosis and treatment of ovarian cancer. CA Cancer J Clin, 6, 183-203.
7 Jemal A, Siegel R, Ward E, et al (2009). Cancer statistics. CA Cancer J Clin, 59, 225-49.   DOI   ScienceOn
8 Johannes L (2010). Test to help determine if ovarian masses are cancer. The Wall Street Journal.
9 Konig B, Gratzel M (1993). Detection of human T-lymphocytes with a piezoelectric immunosensor. Anal Chim Acta, 281, 13-8.   DOI
10 Sauerbrey G (1959). The use of quartz oscillators for weighing layers and for micro-weighing. Zeitschrift for Physik A Hadrons and Nuclei, 155, 206-22.   DOI