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http://dx.doi.org/10.7314/APJCP.2012.13.5.1793

Neurotrophic Artemin Promotes Motility and Invasiveness of MIA PaCa-2 Pancreatic Cancer Cells  

Meng, Ling-Xin (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University)
Chi, Yu-Hua (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University)
Wang, Xiang-Xu (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University)
Ding, Zhao-Jun (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University)
Fei, Li-Cong (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University)
Zhang, Hong (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University)
Mou, Ling (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University)
Cui, Wen (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University)
Xue, Ying-Jie (Department of Oncology, Affiliated Rizhao People's Hospital, Jining Medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.5, 2012 , pp. 1793-1797 More about this Journal
Abstract
Objective: To analyze the capacity of neurotrophic artemin to promote the motility and invasiveness of MIA PaCa-2 pancreatic cancer cells. Methods: MIA PaCa-2 was cultured in vitro and studied using transwell chambers for motility and invasiveness on treatment with different concentrations of aArtemin or its receptor $GFR{\alpha}3$ were also determined. Expression of matrix metalloproteinase-2 (MMP-2) and epithelial cadherin (E-cadherin) was quantified using RT-PCR and Western blotting. Results: MIA PaCa-2 pancreatic cancer cell motility and invasiveness was significantly increased with artemin and its receptor $GFR{\alpha}3$ with dose dependence (P<0.01). MMP-2 production was also significantly increased (t = 6.35, t = 7.32), while E-cadherin was significantly lowered (t = 4.27, t = 5.61) (P <0.01). Conclusion: Artemin and its receptor $GFR{\alpha}3$ can promote pancreatic cancer cell motility and invasiveness and contribute to aggressive behavior. The mechanism may be related to increased expression of MMP-2 molecule and down-regulation of E-cadherin expression.
Keywords
Artemin; invasiveness; motility; pancreatic cancer; MMP-2; E-cadherin;
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