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http://dx.doi.org/10.7314/APJCP.2012.13.4.1617

Gold Nanoparticles Induce Apoptosis in MCF-7 Human Breast Cancer Cells  

Selim, Manar E. (Zoology Department, Faculty of Science, King Saud University)
Hendi, Awatif A. (Physics Department, Faculty of Science, King Saud University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.4, 2012 , pp. 1617-1620 More about this Journal
Abstract
Background: Gold nanoparticles have recently been investigated with respect to biocompatibility according to their interactions with cells. The purpose of this study was to examine cytotoxicity and apoptosis induction by well-characterized gold nanoparticles in human breast epithelial MCF-7 cells. Methods: Apoptosis was assessed by TUNEL, cytotoxicity by MTT assay and caspase 3, 9, p53, Bax and Bcl expression by real-time PCR assays. Results: Gold nanoparticles at up to $200\;{\mu}g/mL$ for 24 hours exerted concentration-dependent cytotoxicity and significant upregulation of mRNA expression of p53, bax, caspase-3 & caspase-9, whereas expression of antiapoptotic bcl-2 was down-regulated. Conclusion: To the best of our knowledge this is the first report showing that gold nanoparticles induce apoptosis in MCF-7cells via p53, bax/bcl-2 and caspase pathways.
Keywords
Gold nanoparticles; MCF-7; apoptosis; cancer;
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