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http://dx.doi.org/10.7314/APJCP.2012.13.4.1349

Up-regulation of Thy-1 Promotes Invasion and Metastasis of Hepatocarcinomas  

Cheng, Bian-Qiao (Department of Hepatology Center, Fuzhou General Hospital, Nanjing Military Area Command)
Jiang, Yi (Department of Hepatology Center, Fuzhou General Hospital, Nanjing Military Area Command)
Li, Dong-Liang (Department of Hepatology Center, Fuzhou General Hospital, Nanjing Military Area Command)
Fan, Jing-Jing (Department of Hepatology Center, Fuzhou General Hospital, Nanjing Military Area Command)
Ma, Ming (Department of Hepatology Center, Fuzhou General Hospital, Nanjing Military Area Command)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.4, 2012 , pp. 1349-1353 More about this Journal
Abstract
Increasing evidence has revealed that thy-1 was a potential stem cell marker of liver cancer, but no data have been shown on how thy-1 regulates the pathophysiology of liver cancer, such as proliferation, apoptosis, invasion and migration. We previously demonstrated that thy-1 was expressed in about 1% of hepg2 cells, thy-1+hepg2 cells, but not thy-1-, demonstrating high tumorigenesis on inoculation $0.5{\times}10^5$ cells per BACA/LA mouse after 2 months. In the present study, our results showed that higher expression of thy-1 occurs in 72% (36/50 cases) of neoplastic hepatic tissues as compared to 40% (20/50 cases) of control tissues, and the expression of thy-1 is higher in poorly differentiated liver tumors than in the well-differentiated ones. In addition, thy-1 expression was detected in 85% of blood samples from liver cancer patients, but none in normal subjects or patients with cirrhosis or hepatitis. There was a significant negative correlation between thy-1expression and E-cadherin expression (a marker of invasion and migraton), but not between thy-1 expression and AFP expression in all the liver cancer and blood samples. We further investigated the relationship between thy-1 and E-cadherin in liver cancer hepg2 cell line which was transfected with pReceiver-M29/thy-1 eukaryotic expression vector followed by aspirin treatment. Lower expression of E-cadherin but higher expressions of thy-1 were detected in hepg2 cells transfected with pReceiver-M29/thy-1. Taken together, our study suggested that thy-1 probably regulates liver cancer invasion and migration.
Keywords
Invasion; migration; thy-1; hepatocarcinomas;
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