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http://dx.doi.org/10.7314/APJCP.2012.13.1.027

Expression of VEGF-C/VEGFR-3 in Human Laryngeal Squamous Cell Carcinomas and its Significance for Lymphatic Metastasis  

Wang, Zhongliang (Department of Otolaryngology-Head and Neck Surgery, Subei People's Hospital of Jiangsu Province)
Chen, Yao (Department of Human Anatomy, West China of Preclinical and Forensic Medicine, Sichuan University)
Li, Xiaofeng (Department of Otolaryngology-Head and Neck Surgery, Subei People's Hospital of Jiangsu Province)
Xu, Li (Department of Otolaryngology-Head and Neck Surgery, Subei People's Hospital of Jiangsu Province)
Ma, Wei (Department of Otolaryngology-Head and Neck Surgery, Subei People's Hospital of Jiangsu Province)
Chang, Lingmei (Department of Otolaryngology-Head and Neck Surgery, Subei People's Hospital of Jiangsu Province)
Ju, Funian (Department of Otolaryngology-Head and Neck Surgery, Subei People's Hospital of Jiangsu Province)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.1, 2012 , pp. 27-31 More about this Journal
Abstract
Objectives: Expression of vascular endothelial growth factor C (VEGF-C)and vascular endothelial growth factor feceptor-3 (VEGFR-3) in laryngeal squamous carcinoma and its relationship to lymph node metastasis were investigated. Methods: VEGF-C and VEGFR-3 gene expression in 30 cases of normal laryngeal mucosa tissue (NLM), primary laryngeal carcinoma cell carcinomas (PLC) and cervical lymph nodes (CLN) was examined by reverse transcription polymerase chain reaction (RT-PCR). Protein levels of VEGF-C expression were determined by immunohistochemical staining in 60 cases of PLC. Results: Expression of VEGF-C and VEGFR-3 different among NLM, PLC and CLN in the same patient. In PLC, expression was significantly higher in lymph node positive group than in the lymph node negative group and associated with histological grade of differentiation; Expression of VEGF-C and VEGFR-3 was not linked with age, sex, site or T stage. Conclusions: A close correlation was found between VEGF-C/VEGFR-3 expression and lymph node metastasis in PLC, suggesting a role in metastasis of laryngeal carcinomas.
Keywords
VEGF-C; VEGFR-3; laryngeal squamous cell carcinomas; lymph node metastasis;
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