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http://dx.doi.org/10.7314/APJCP.2012.13.1.205

Paris polyphylla Smith Extract Induces Apoptosis and Activates Cancer Suppressor Gene Connexin26 Expression  

Li, Fu-Rong (School of Pharmaceutical Science, Taishan Medical University)
Jiao, Peng (Institute of Basic Medical Sciences, Taishan Medical University)
Yao, Shu-Tong (Department of Pathophysiology, Taishan Medical University)
Sang, Hui (Department of Pathophysiology, Taishan Medical University)
Qin, Shu-Cun (Institute of Basic Medical Sciences, Taishan Medical University)
Zhang, Wei (School of Pharmaceutical Science, Taishan Medical University)
Zhang, Ya-Bin (Department of Pathophysiology, Taishan Medical University)
Gao, Lin-Lin (Department of Pathophysiology, Taishan Medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.1, 2012 , pp. 205-209 More about this Journal
Abstract
Background: The inhibition of tumor cell growth without toxicity to normal cells is an important target in cancer therapy. One possible way to increase the efficacy of anticancer drugs and to decrease toxicity or side effects is to develop traditional natural products, especially from medicinal plants. Paris polyphylla Smith has shown anti-tumour effects by inhibition of tumor promotion and inducement of tumor cell apoptosis, but mechanisms are still not well understood. The present study was to explore the effect of Paris polyphylla Smith extract (PPSE) on connexin26 and growth control in human esophageal cancer ECA109 cells. Methods: The effects of PPSE on Connexin26 were examined by RT-PCR, western blot and immunofluorescence; cell growth and proliferation were examined by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. Results: PPSE inhibited the growth and proliferation on esophageal cancer ECA109 cells, while increasing the expression of connexin26 mRNA and protein; conversely, PPSE decreased Bcl-2 and increased Bad. Conclusion: This study firstly shows that PPSE can increase connexin26 expression at mRNA and protein level, exerting anti-tumour effects on esophageal cacner ECA109 cells via inhibiting cell proliferation and inducing cell apoptosis.
Keywords
Connexin; Paris polyphylla smith; esophageal cancer; apoptosis;
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