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http://dx.doi.org/10.7314/APJCP.2012.13.1.181

Association Between p16, hMLH1 and E-cadherin Promoter Hypermethylation and Intake of Local Hot Salted Tea and Sun-dried Foods in Kashmiris with Gastric Tumors  

Mir, Manzoor R. (Division of Veterinary Biochemistry, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir)
Shabir, Nadeem (Division of Veterinary Biochemistry, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir)
Wani, Khursheed A. (Department of General Surgery, Sher-I-Kashmir Institute of Medical Sciences)
Shaffi, Sheikh (Division of Veterinary Biochemistry, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir)
Hussain, Ishraq (Division of Veterinary Biochemistry, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir)
Banday, Manzoor A. (Department of Medical Oncology, Sher-I-Kashmir Institute of Medical Sciences)
Chikan, Naveed A. (Department of Medical Biotechnology, Vellore Institute of Technology)
Bilal, S. (Division of Veterinary Biochemistry, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir)
Aejaz, Sheikh (Department of Medical Oncology, Sher-I-Kashmir Institute of Medical Sciences)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.1, 2012 , pp. 181-186 More about this Journal
Abstract
The aim of this study was to evaluate the methylation status of three important cancer related genes viz. p16, E-cadherin and hMLH1 promoters and to associate the findings with specific dietary habits in Kashmiris, a culturally distinct population in India, with gastric cancer. The study subjects were divided into three age groups viz. 0-30yrs ($1^{st}$), 31-60yrs ($2^{nd}$) and 61-90yrs ($3^{rd}$). A highly significant association between the intake of local hot salted tea in $2^{nd}$ (p=0.001) and $3^{rd}$ (p=0.009) age groups was observed with the promoter hypermethylation of E cadherin. Again a highly significant association between the aberrant methylation of hMLH1 (p=0.000) and p16 (p=0.000) promoters and the intake of local hot salted tea was observed in the $2^{nd}$ age group of gastric cancer patients. The intake of sun-dried food was also significantly associated with the promoter hypermethylation of E cadherin (p=0.003) and p16 (p=0.015) genes in $3^{rd}$ age group. The results of the present study suggest a close association between the aberrant methylation of p16, E-cadherin and hMLH1 promoters and the intake of local hot salted tea and sun-dried foods in Kashmiri population.
Keywords
Gastric carcinoma; Kashmir; methylation;
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1 Wijnhoven BPL, Dinjens WNM (2000). E-cadherin-catenin cell-cell adhesion complex and human cancer. Br J Surg, 87, 992-1005.   DOI
2 Rocco JW, Sidransky D(2001). p16 (MTS-1/CDKN2/INK4a) in cancer progression. Exp Cell Res, 264, 42-55 .   DOI
3 Sakata K, Tamura G, Endoh Y, et al (2005). Hypermethylation of the hMLH1 gene promoter in solitary and multiple gastric cancers with microsatellite instability. Br J Cancer, 86, 564-67.   DOI
4 Salam I, Hussain S, Mir MM, et al (2009). Aberrant promoter methylation and reduced expression of p16 gene in esophageal squamous cell carcinoma from Kashmir valley: a high-risk area. Mol Cell Biochem, 332, 51-8.   DOI
5 Sanchez Cespedes M, Esteller M (2000). Gene promoter hypermethylation in tumors and serum of head and neck cancer patients. Cancer Res, 60, 892-95.
6 Santos Reboucas CB, Pimentel MM (2007). Implication of abnormal epigenetic patterns for human diseases. Eur J Hum Genet, 15, 10-17.   DOI
7 Siddiqi M, Preussmann R (1989). Esophageal cancer in Kashmir -an assessment. J Cancer Res Clin Oncol, 115, 111-17.   DOI
8 Siddiqi M, Tricker AR, et al (1988). Formation of N-nitroso compounds under simulated gastric conditions from Kashmir foodstuffs. Cancer Lett, 39, 259-65.   DOI
9 Siddiqi M, Tricker AR, et al (1988). The occurrence of preformed N-nitroso compounds in food samples from a high risk area of esophageal, cancer in Kashmir, India. Cancer Lett, 39, 37-43.   DOI
10 Suzuki FH (1999). Distinct methylation pattern and microsatellite instability in sporadic gastric cancer. Int J Cancer, 83, 309-13.   DOI
11 Takeichi M (1991). Cadherin cell adhesion receptors as a morphogenetic regulator. Science, 251, 1451-55.   DOI
12 Tamura YG, Ajioka Y, et al (2000). Frequent hypermethylation of the hMLH1 gene promoter in differentiated-type tumors of the stomach with the gastric foveolar phenotype. Am J Pathol, 157, 717-22.   DOI
13 Ksiaa F, Ziadi S, Amara K, et al (2009). Biological significance of promoter hypermethylation of tumor-related genes in patients with gastric carcinoma. Clinica Chimica Acta, 404, 128-33.   DOI
14 Lawson TA, Gingell R (1981). Methylation of hamster DNA by the carcinogen N-nitroso-bis (2-oxopropyl)amine. Cancer Lett, 11, 251-55.   DOI
15 Leal MF, Lima EM, et al (2007). Promoter hyper-methylation of CDH1, FHIT, MTAP and PLAGL1 in gastric adenocarcinoma in individuals from Northern Brazil. World J Gastroenterol, 13, 2568-74.   DOI
16 Leung KH, Archer MC (1985). Mechanism of DNA methylation by N-nitroso(2oxopropyl)propylamine. Carcinogenesis, 6, 189-91 .   DOI
17 Leung SY, Yuen ST (1999). hMLH1 promoter methylation and lack of hMLH1 expression in sporadic gastric carcinomas with high-frequency microsatellite instability. Cancer Res, 59, 159-64.
18 Levenberg S, Yarden A, Kam Z, Geiger B (1999). p27 is involved in N-cadherin-mediated contact inhibition of cell growth and S-phase entry. Oncogene, 18, 869-76.   DOI
19 Malik GM, Mubarik M, Kadla SA, Durrani HA (2009). Gastric cancer profile in kashmiri population with special dietary habits. Diagn Ther Endosc, 6, 83-6.   DOI
20 Milligan JR, Hirani Hojatti S, Catz-Biro L, Archer MC (1989). Methylation of DNA by three N-nitroso compounds: Evidence for sequence specific methylation by a common intermediate. Chem Biol Interact, 72, 175-89.   DOI
21 Nan HM, Song YJ, Yun HY, et al (2005). Effect on dietary intake and genetic factors on hypermethylation of the hMLHI1 gene promoter in gastric cancer. World J gasteroenterol, 11, 3834-41.   DOI
22 Chan AO, Lam SK, Wong BC, et al (2002). Promoter methylation of E-cadherin gene in gastric mucosa associated with Helicobacter pylori infection and in gastric cancer. Gut, 52, 502-06.
23 Ngoan LT, Mizoue T, Fujino Y, et al (2002). Dietary factors and stomach cancer mortality. Br J Cancer, 87, 37-42.   DOI
24 Pignatelli M, Vessey CJ (1994). Adhesion molecules: novel molecular tools in tumour pathology. Hum Pathol, 25, 849-56   DOI
25 Carvalho B, Pinto M, Cirnes L, et al (2005). Concurrent hypermethylation of gene promoters is associated with a MSI-H phenotype and diploidy in gastric carcinomas. Eur J Cancer, 39, 1222-27 .   DOI
26 Cloutier JF, Drouin R, Castonguay A, et al(1999). Treatment of human cells with N-Nitroso(acetoxymethyl)methylamine: distribution patterns of piperidine-sensitive DNA damage at the nucleotide level of resolution are related to the sequence context. Chem Res Toxicol, 12, 840-49.   DOI
27 El Naggar AK, Lai S, Clayman G, et al (1997). Methylation, a major mechanism of p16/CDKN2 gene inactivation in head and neck squamous carcinoma. Am J Pathol, 151, 1767-74.
28 Fleisher AS, Esteller M, Tamura G, et al (1999). Hypermethylation of the hMLH1 gene promoter in human gastric cancers with microsatellite instability. Cancer Res, 59, 1090-95 .
29 Heinzel PA, Balaram P, Bernard HU, et al (1996). Mutations and polymorphisms in the p53, p21 and p16 genes in oral carcinomas of Indian betel quid chewers. Int J Cancer, 68, 420-23 .   DOI
30 Hirohashi S (1998). Inactivation of the E-cadherin-mediated cell adhesion system in human cancers. Am J Pathol, 153, 333-39 .   DOI
31 Cairns P, Polascik TJ, Eby Y, et al (1995). Frequency of homozygous deletion at p16/CDKN2 in primary human tumours. Nat Genet, 11, 210-12 .   DOI
32 Huang Q, Ai L, Zhang ZY, et al (2004). Promoter hypermethylation and protein expression of the p16 gene: analysis of 43 cases of B-cell primary gastric lymphomas from China. Mod Patho, 17, 416-22 .   DOI
33 Kang GH, Shim YH, Yung HY, et al (2001).CpG Island methylation in premalignant stages of gastric carcinoma. Cancer Res, 61, 2847-51.
34 Khuroo MS, Zargar SA, Mahajan R, et al (1992). High incidence of esophageal and gastric cancer in Kashmir in a population with special personal and dietary habits. Gut, 33, 11-15.   DOI
35 Baylin SB, Herman JG, Graff JR, et al (1998). Alterations in DNA methylation: a fundamental aspect of neoplasia. Adv Cancer Res, 72, 141-96 .