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http://dx.doi.org/10.7314/APJCP.2012.13.1.135

Apoptin Induces Apoptosis in Human Bladder Cancer EJ and BIU-87 Cells  

Zhan, Hui (Department of Urology, the Second Affiliated Hospital of Kunming Medical University)
Wang, Jian-Song (Department of Urology, the Second Affiliated Hospital of Kunming Medical University)
Wang, Hai-Feng (Department of Urology, the Second Affiliated Hospital of Kunming Medical University)
Zuo, Yi-Gang (Department of Urology, the Second Affiliated Hospital of Kunming Medical University)
Wang, Chun-Hui (Department of Urology, the Second Affiliated Hospital of Kunming Medical University)
Ding, Ming-Xia (Department of Urology, the Second Affiliated Hospital of Kunming Medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.1, 2012 , pp. 135-138 More about this Journal
Abstract
Objective: To investigate whether apoptin is a apoptosis-inducing protein with a potential for bladder cancer therapy. Methods: We constructed a PCDNA3/Apoptin eukaryotic expression vector, and transfected this vector into bladder cancer cell lines BIU-87 and EJ, then observed the results by RT-PCR, transmission electron microscopy, MTT assay and the flow cytometry (TUNEL method). Results: PCDNA3/Apoptin successfully induced a high level apoptosis in both bladder cancer cell lines, compared with the controls (p<0.05). Conclusions: Apoptin can induce high level apoptosis in human bladder cancer EJ and BIU-87 cells, which suggests a potential for human bladder cancer therapy.
Keywords
Apoptin; urinary bladder neoplasms; apoptosis; therapy;
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