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http://dx.doi.org/10.7314/APJCP.2012.13.10.5149

Aberrant Expression of E-cadherin in Lung Tissues of Patients with Probable Lung Cancer  

Yuan, Yu-Lin (Department of Clinical Laboratory, The Second Xiangya Hospital, Central South University)
Wang, Yu-Ming (Department of Clinical Laboratory, The First Affilliated Hospital of Kunming Medical University)
Liu, Hua (Department of Clinical Laboratory, The First Affilliated Hospital of Kunming Medical University)
Qin, Gui-Fang (Department of Clinical Laboratory, The People's Hospital of Guangxi Zhuang Autonomous Region)
Tang, Ai-Guo (Department of Clinical Laboratory, The Second Xiangya Hospital, Central South University)
Duan, Yong (Department of Clinical Laboratory, The First Affilliated Hospital of Kunming Medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.10, 2012 , pp. 5149-5153 More about this Journal
Abstract
Introduction: This study assessed the relationship of E-cadherin mRNA and protein expression with the diagnosis of lung cancer with the aim of providing an auxiliary diagnostic method. Methods: Semi-quantitative nested RT-PCR and western blotting were applied to detect E-cadherin mRNA transcripts and protein, respectively, in 30 cases of diagnostic lung cancer, 30 cases of clinically suspected patients with lung cancer and 30 cases of other disease. Immunohistochemical staining was also used to detect E-cadherin. Results: Remarkably decreased levels of relative E-cadherin mRNA value and increased E-cadherin protein negativity were observed in probable lung cancer, when compared with possible lung cancer and others. With a threshold of 1.45, relative E-cadherin mRNA value showed a sensitivity of 90% and a specifity of 83% for the diagnosis of lung cancer. The combination of decreased relative E-cadherin mRNA value and negative E-cadherin protein increased the specificity and sensitivity. Conclusion: These data suggest that Chinese patients with diagnostic lung cancer have similar decreased levels of relative E-cadherin mRNA and E-cadherin protein value in the lung cancer tissues as in lung cancer patients in other countries. Measurement of relative E-cadherin mRNA and protein values in lung cancer tissues has potential for lung cancer diagnosis.
Keywords
Aberrant expression; E-cadherin; lung cancer diagnosis; lung tissue;
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