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Effect of Solvent on the Surface of Protein Chip Plate  

현준원 (단국대학교 자연과학부)
윤미영 (단국대학교 자연과학)
안상민 (단국대학교 자연과학)
노승정 (단국대학교 자연과학)
허영덕 (단국대학교 자연과학)
박헌용 (단국대학교 자연과학)
송예신 (단국대학교 자연과학)
피재호 (단국대학교 자연과학)
김경례 ((주)휴시피)
Publication Information
Journal of the Korean institute of surface engineering / v.37, no.2, 2004 , pp. 76-79 More about this Journal
Abstract
Nickel chloride coated protein chip plate was developed by using a spin coating method. The ability of histidine tagged protein adsorption was investigated at various solvents. The surface of plate has a large aggregated nickel complex with high density in water. However, the surface of plate has a very small size of aggregated nickel complex with low density in isopropanol. The ability of protein adsorption decreased as increasing the size of alkyl chain in various alcohol solvents. The mechanism on the ability of protein adsorption at the plate surface is discussed.
Keywords
Protein chip plate; Sol-gel methods; NiCl$_2$ Film; His-tagged;
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