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http://dx.doi.org/10.17555/jvc.2018.12.35.6.273

Molecular Detection of Mycoplasma felis Infection in a Cat with Respiratory Symptoms  

Lee, Hyun-A (Center for Animal Resources Development, Wonkwang University)
Hong, Sunhwa (Center for Animal Resources Development, Wonkwang University)
Chung, Yungho (Department of Companion Animal and Animal Resources Science, Joongbu University)
Kim, Okjin (Center for Animal Resources Development, Wonkwang University)
Publication Information
Journal of Veterinary Clinics / v.35, no.6, 2018 , pp. 273-275 More about this Journal
Abstract
A 6-month-old male cat was presented for investigation of depression, loss of appetite, dehydration, pale conjunctival mucous membrane, weight loss, fast heart and respiratory rates, nasal discharge and cough. Nasal swabs collected from the studied cat. As the results of bacterial culture with nasal swabs, it was suspected with Mycoplasma spp. Also, Mycoplasma species was detected by the PCR reaction with Mycoplasma genus primers. At species PCR assay, the specimens evaluated for the presence of M. felis, M. arginini, M. gateae, and Acholeplasma laidlawii and the result was visualization of bands from 238 bp in agarose gel 1.5% showing M. felis amplicons in samples. In conclusion, we detected M. felis in a cat with respiratory disease. PCR was able to detect successfully M. felis infection in cats.
Keywords
PCR; Mycoplasma; M. felis; respiratory; cat;
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