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http://dx.doi.org/10.17555/jvc.2017.06.34.3.172

Fucoidan Suppresses Prostaglandin E2 Production and Akt Activation in Lipopolysaccharide-Stimulated Porcine Peripheral Blood Mononuclear Cells  

Park, Geon-Tae (Department of Veterinary Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University)
Ahn, Changhwan (Department of Veterinary Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University)
Kang, Byeong-Teck (Department of Veterinary Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University)
Kang, Ji-Houn (Department of Veterinary Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University)
Jeung, Eui-Bae (Department of Veterinary Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University)
Yang, Mhan-Pyo (Department of Veterinary Medicine, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University)
Publication Information
Journal of Veterinary Clinics / v.34, no.3, 2017 , pp. 172-177 More about this Journal
Abstract
Fucoidan, a cell wall polysaccharide found in the brown seaweed, is reported to have broad-spectrum biological activities. The objectives of this study were to examine the effect of fucoidan on prostaglandin $E_2$ ($PGE_2$) and cyclooxygenase-2 (COX-2) expression in lipopolysaccharide (LPS)-stimulated porcine peripheral blood mononuclear cells (PBMCs) and to determine whether these effects are involved in Akt activation. The levels of $PGE_2$ production in the culture supernatants from PBMCs were determined by the enzyme-linked immunosorbent assay (ELISA) kit and the levels of COX-2 mRNA were measured by real time polymerase chain reaction (RT-PCR). Akt activity was determined by Western blot analysis. Fucoidan in LPS-$na{\ddot{i}ve}$ PBMCs has no effect on $PGE_2$ production and COX-2 mRNA expression. Furthermore, fucoidan does not affect Akt activation in LPS- $na{\ddot{i}ve}$ PBMCs. However, $PGE_2$ production and COX-2 mRNA expression on PBMCs were remarkably enhanced by LPS stimulation. Akt activity was also increased by LPS. Increasing effects of $PGE_2$ production and COX-2 mRNA expression in PBMCs induced by LPS were suppressed by addition of fucoidan. In addition, fucoidan reduced an increase in Akt activity in LPS-stimulated PBMCs. These results suggested that fucoidan exerts potent anti-inflammatory properties by suppression of $PGE_2$ production, COX-2 mRNA expression and Akt activation in LPS-stimulated PBMCs.
Keywords
fucoidan; Akt; anti-inflammation; PBMCs; $PGE_2$; COX-2; porcine;
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