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http://dx.doi.org/10.17555/jvc.2015.08.32.4.289

Effects of Fucoidan on Nitric Oxide Production and Activator Protein-1 Activation in Lipopolysaccharide-Stimulated Porcine Peripheral Blood Mononuclear Cells  

Park, Jongchan (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
Ahn, Changhwan (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
Kang, Byeong-Teck (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
Kang, Ji-Houn (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
Jeung, Eui-Bae (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
Yang, Mhan-Pyo (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
Publication Information
Journal of Veterinary Clinics / v.32, no.4, 2015 , pp. 289-294 More about this Journal
Abstract
Fucoidan which is sulfated polysaccharide extracted from brown seaweed has a wide variety of internal biological activities. The objectives of this study were to examine the effect of fucoidan on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated porcine peripheral blood mononuclear cells (PBMCs) and to investigate whether this effect is involved in the expression of inducible nitric oxide synthase (iNOS) and the activation of activator portein-1 (AP-1). The levels of NO production and AP-1 activity in the culture supernatants from porcine PBMCs were measured by the enzyme-linked immunosorbent assay and the levels of iNOS and AP-1 mRNA were determined by real time polymerase chain reaction. Fucoidan in LPS-naïve PBMCs has no effects on the production of NO and activity of AP-1. Expressions of iNOS and AP-1 mRNA in LPS-naïve PBMCs were also not affected by treatment of fucoidan. However, NO production, AP-1 activity and expressions of iNOS and AP-1 mRNA were dramatically increased in PBMCs stimulated with LPS. Enhancing effects of NO production and AP-1 activity in PBMCs induced by LPS were reduced by addition of fucoidan. Fucoidan also inhibited an increase in expressions of iNOS and AP-1 mRNA in LPS-stimulated PBMCs. These results suggested that fucoidan exerts anti-inflammatory effect by down-regulating production of NO via suppressing expression of iNOS and activity of AP-1 in LPS-stimulated porcine PBMCs.
Keywords
fucoidan; AP-1; anti-inflammation; PBMCs; NO; iNOS; porcine;
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