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http://dx.doi.org/10.17555/jvc.2015.04.32.2.135

Effect of trans-10, cis-12 Conjugated Linoleic Acid on Calcium-Dependent Reactive Oxygen Species and Nitric Oxide Production and Nuclear Factor-${\kappa}B$ Activation in Lipopolysaccharide-Stimulated RAW 264.7 Cells  

Choi, Tae-Won (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
Kang, Byeong-Teck (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
Kang, Ji-Houn (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
Yang, Mhan-Pyo (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
Publication Information
Journal of Veterinary Clinics / v.32, no.2, 2015 , pp. 135-140 More about this Journal
Abstract
Trans-10, cis-12-conjugated linoleic acid (t10c12-CLA) has been shown to participate in the regulation of anti-inflammatory effects. The objectives of this study were to examine the effects of t10c12-CLA on reactive oxygen species (ROS) and nitric oxide (NO) production and nuclear factor-kappaB (NF-${\kappa}B$) activation in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and to determine whether these effects were associated with change of intracellular calcium ion ($Ca^{2+}$). ROS production was increased in LPS-stimulated RAW 264.7 cells, and this effect was suppressed by 1,2-bis-(o-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester (BAPTA/AM), a calcium chelator. t10c12-CLA suppressed ROS production in LPS-stimulated RAW 264.7 cells, which was further more decreased by treatment with BAPTA/AM. These indicated that t10c12-CLA decreases $Ca^{2+}$-dependent ROS production in LPS-stimulated RAW 264.7 cells. Similarly, NF-${\kappa}B$ p65 DNA binding activity and NO production were decreased by treatment with either t10c12-CLA, BAPTA/AM, or t10c12-CLA and BAPTA/AM combination. However, there were no differences between t10c12-CLA and BAPTA/AM treatment in NO production of LPS-stimulated RAW 264.7 cells. These data indicate that t10c12-CLA inhibits the increases in ROS and NO production and the NF-${\kappa}B$ activation in LPS-stimulated condition. These results suggested that CLA exerts potent anti-inflammatory effects by suppression of LPS-induced ROS and NO production, and NF-${\kappa}B$ activationn via $Ca^{2+}$-dependent pathway.
Keywords
conjugated linoleic acid (CLA); lipopolysaccharide (LPS); reactive oxygen species (ROS); nuclear factor-kappaB (NF-${\kappa}B$); nitric oxide (NO); calcium ion;
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