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Evaluation for Protective Effect of CPV-2 and CPV-2b Vaccines against a Korean CPV-2a Isolate in Pups  

Jeoung, Seok-Young (Gangnam Animal Clinic)
Yi, Jun-Seok (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
Kim, Hyun-Tae (College of Veterinary Medicine, Seoul National University)
Kim, Doo (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
Publication Information
Journal of Veterinary Clinics / v.31, no.5, 2014 , pp. 361-366 More about this Journal
Abstract
The aim of this study was to determine if vaccines containing CPV-2 or CPV-2b provided protection against challenge with a recent Korean CPV-2a isolate. Twenty mongrel pups aged 9 weeks old were used. The commercial CPV-2 or CPV-2b vaccines were administered to each of the 8 pups thrice every 3 weeks, respectively. Two weeks after the last vaccination, all pups were challenged with CPV-2a (VR00174 strain) $1{\times}10^6\;TCID_{50}$. Clinical signs, fecal excretion of challenged CPV, and serological response of pups were observed for 2 weeks after challenge. All vaccinated pups did not display any clinical signs of disease after challenge with Korean CPV-2a isolate, whereas all non-vaccinated pups exhibited mucoid or hemorrhagic diarrhea, vomiting and anorexia. In all non-vaccinated pups, the virus could be detected in feces from 4 days after challenge, whereas in vaccinated pups, no evidence of viral excretion could be detected. Two of 4 non-vaccinated pups died 6 days after the challenge. This study showed that the two commercial CPV-2 and CPV-2b vaccines were effective in preventing infection and/or disease caused by the Korean CPV-2a isolate.
Keywords
canine parvovirus; CPV-2a; vaccines; protection; Korea;
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