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Effect of ${\beta}$-glucan Originated from Aureobasidium on the Dermal Wound Repair in Vitro Model  

Lee, Jung-Woo (College of Veterinary Medicine, Kyungpook National University)
Kwon, Young-Sam (College of Veterinary Medicine, Kyungpook National University)
Jang, Kwang-Ho (College of Veterinary Medicine, Kyungpook National University)
Publication Information
Journal of Veterinary Clinics / v.30, no.6, 2013 , pp. 403-408 More about this Journal
Abstract
The objective of the present study is to detect the effect of ${\beta}$-glucan originated from Aureobasidium on the proliferation and collagen production in human dermal fibroblast cells with wound repopulation in vitro. The proliferative effects were assessed using a MTT assay as well as cell counts at 24 and 48 hr after treatment. Hydroxyproline was measured as an index of procollagen production with reverse-phase high pressure liquid chromatography. Oncostatin M was used as a reference agent. In glucagon treated group, dose-dependent and significant increase of optical density or fibroblast cell numbers was demonstrated, when compared with those of control from 0.1 mg/ml concentration. In addition, the numbers of cells which had migrated into the wound defects were more significantly and dose-dependently increased than those of non-treated control. However, no meaningful effects on the procollagen production were observed.
Keywords
${\beta}$-glucan; Aureobasidium; nude mouse; wound management; infection;
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