Browse > Article

Effects of Vitamin C on Oxidative Stress Due to Anesthesia and Surgical Trauma in Dogs  

Choi, Kyeong-Ha (College of Veterinary Medicine, Chungnam National University)
Lee, Jae-Yeon (College of Veterinary Medicine, Chungnam National University)
Jeong, Seong-Mok (College of Veterinary Medicine, Chungnam National University)
Joo, Gregory K. (Cottman Animal Hospital)
Kim, Myung-Cheol (College of Veterinary Medicine, Chungnam National University)
Publication Information
Journal of Veterinary Clinics / v.28, no.5, 2011 , pp. 473-478 More about this Journal
Abstract
The study evaluated the antioxidant effects of ascorbic acid (AA) against oxidative stress during laparotomy in dogs under general anesthesia using isoflurane. Twelve dogs were randomly assigned to either the ascorbic acid group (AAG) or the sham group (SG). AAG received intravenous dosage of 100 mg/animal of standard AA 10 minutes before anesthesia. Plasma levels of cortisol, glucose, total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) were measured. Cortisol level increased significantly with time in both groups (p < 0.05). The change of glucose levels was not significantly different between both groups. TOS and OSI increased significantly with time in the SG (p < 0.05), whereas it did not significantly change with time in the AAG. The TOS and OSI of the AAG were significantly lower than in the SG (p < 0.05). However, TAS did not significantly change between both groups. The results suggest that the decrease of TOS in the AAG might be related to the conversion of antioxidants to oxidants. The decrease of OSI indicates that the decrease of reactive oxygen species (i.e., oxidative stress) produced at the site of injury is able to decrease surgical side effects of abnormal blood circulation, organ failure, and inflammation. Therefore, AA can be used to protect a surgical patient from oxidative stress in canine laparotomy.
Keywords
laparotomy; ascorbic acid; antioxidant; cortisol; dog;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Bartels M, Vilsendorf AM, Kassahun1 WT, Gerstenbergk B, Engelhart K, Vilsendorf EM, Faber S, Biesalski HK. Protective effect of antioxidative vitamins against lipid peroxidation in liver ischemia and reperfusion - an animal experimental study. EXCLI J 2007; 6: 152-166.
2 Baysal Z, Togrul T. Evaluation of total oxidative and antioxidative status in pediatric patients undergoing laparoscopic surgery. J Pediatr Surg. 2009; 44: 1367-1370.   DOI   ScienceOn
3 Behrendt D, Beltrame J, Hikiti H, Wainstein M, Kinlay S, Selwyn AP, Ganz P, Fang JC. Impact of coronary endothelial function on the progression of cardiac transplant-associated arteriosclerosis: effect of anti-oxidant vitamins C and E. J Heart Lung Transplant 2006; 25: 426-433.   DOI   ScienceOn
4 Berger MM, Shenkin A. Update on clinical micronutrient supplementation studies in the critically ill. Curr Opin Clin Nutr Metab Care 2006; 9: 711-716.   DOI   ScienceOn
5 Elsa A, Ubandawaki S. Ketamine anaesthesia following premedication of rabbits with vitamin C. J Vet Sci 2005; 6: 239-241.
6 De Vries AP, Oterdoom LH, Gans RO, Bakker SJ. Supplementation with anti-oxidants Vitamin C and E decreases cyclosporine A trough-levels in renal transplant recipients. Nephrol Dial Transplant 2006; 21: 231-232.   DOI   ScienceOn
7 Dodam JR, Kruse-Elliott KT, Aucoin DP, Swanson CR. Duration of etomidate-induced adrenocortical suppression during surgery in dogs. Am J Vet Res 1990; 51: 786-788.
8 Egwu GO, Mshelia GD, Sanni S, Onyeyili PA, Adeyanju GT. The effect of vitamin C at varying times on physiological parameters in rabbits after xylazine anaesthesia. Vet Ital 2011; 47: 97-104.
9 Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem 2004; 37: 277-285.   DOI   ScienceOn
10 Fukushima R, Yamazaki E. Vitamin C requirement in surgical patients. Curr Opin Clin Nutr Metab Care 2010; 13: 669-676.   DOI   ScienceOn
11 Gunel E, Caglayan F, Caglayan O, Dilsiz A, Duman S, Aktan M. Treatment of intestinal reperfusion injury using antioxidative agents. J Pediatr Surg 1998; 33: 1536-1539.   DOI   ScienceOn
12 Gutteridge J, Mitchell J. Redox imbalance in the critically ill. Br Med Bull 1999; 55: 49-75.   DOI   ScienceOn
13 Pirbudak L, Balat O, Cekmen M, Ugur MG, Aygun S, Oner U. Effect of ascorbic acid on surgical stress response in gynecologic surgery. Int J Clin Pract 2004; 58: 928-931.   DOI   ScienceOn
14 Marana E, Scambia G, Maussier ML, Parpaglioni R, Ferrandina G, Meo F, Sciarra M, Marana R. Neuroendocrine stress response in patients undergoing benign ovarian cyst surgery by laparoscopy, minilaparotomy, and laparotomy. J Am Assoc Gynecol Laparosc 2003; 10: 159-165.   DOI   ScienceOn
15 Nathens AB, Neff MJ, Jurkovich GJ, Klotz P, Farver K, Ruzinski JT, Radella F, Garcia I, Maier RV. Randomized, prospective trial of antioxidant supplementation in critically ill surgical patients. Ann Surg 2002; 236: 814-822.   DOI   ScienceOn
16 Papich MG. Handbook of veterinary drugs. 1st ed, Philadelphia: Saunders. 2002: 31.
17 Ryu HY, Lee CJ, Soh DM. Ho Choi, Ihm SH. Effects of vitamin C as antioxidant on recovery of left ventricular function after ischemia and reperfusion in isolated rat heart. Korean J Thorac Cardiovasc Surg 1996; 593-598.
18 Sagsoz N, Kisa U, Apan A. Ischaemia-reperfusion injury of rat ovary and the effects of vitamin C, mannitol and verapamil. Hum Reprod 2002; 17: 2972-2976.   DOI
19 Buyukhatipoglu H, Sezen Y, Yildiz A, Bas M, Kirhan I, Ulas T, Turan MN, Taskin A, Aksoy N. N-acetylcysteine fails to prevent renal dysfunction and oxidative stress after noniodine contrast media administration during percutaneous coronary interventions. Pol Arch Med Wewn 2010; 120: 383-389.
20 Bulger EM, Maier RV. Antioxidants in critical illness. Arch Surg 2001; 136: 1201-1207.   DOI   ScienceOn
21 Caryl Goodyear-Bruch and Janet D Pierce. Oxidative Stress in Critically Ill Patients. Am J Crit Care 2002; 11: 543-551.
22 Lagowska-Lenard M, Stelmasiak Z, Bartosik-Psujek H. Influence of vitamin C on markers of oxidative stress in the earliest period of ischemic stroke. Pharmacol Rep 2010; 62: 751-756.   DOI
23 Sasaki M, Joh T. Oxidative stress and ischemia-reperfusion injury in gastrointestinal tract and antioxidant, protective agents. J Clin Biochem Nutr 2007; 40: 1-12.
24 Trachootham D, Lu W, Ogasawara MA, Nilsa RD, Huang P. Redox regulation of cell survival. Antioxid Redox Signal 2008; 10: 1343-1374.   DOI   ScienceOn
25 Tsuchiya M, Sato EF, Inoue M, Asada A. Open abdominal surgery increases intraoperative oxidative stress: can it be prevented. Anesth Analg 2008; 107: 1946-1952.   DOI
26 Kohen R, Nyska A. Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol 2002; 30: 620-650.   DOI
27 Koksal H, Kurban S. Total oxidant status, total antioxidant status, and paraoxonase and arylesterase activities during laparoscopic cholecystectomy. Clinics (Sao Paulo) 2010; 65: 285-290.   DOI   ScienceOn
28 Lee JI, Son HY, Kim MC. Attenuation of ischemiareperfusion injury by ascorbic acid in the canine renal transplantation. J Vet Sci 2006; 7: 375-379.   DOI   ScienceOn
29 Ullegaddi R, Powers HJ, Gariballa SE. Antioxidant supplementation enhances antioxidant capacity and mitigates oxidative damage following acute ischaemic stroke. Eur J Clin Nutr. 2005; 59: 1367-1373.   DOI   ScienceOn
30 Cuzzocrea S, Riley DP, Caputi AP, Salvemini D. Antioxidant therapy: a new pharmacological approach in shock, inflammation, and ischemia / reperfusion injury. Pharmacol Rev 2001; 53: 135-159.
31 Van Stijn MF, Ligthart-Melis GC, Boelens PG, Scheffer PG, Teerlink T, Twisk JW, Houdijk AP, Van Leeuwen PA. Antioxidant enriched enteral nutrition and oxidative stress after major gastrointestinal tract surgery. World J Gastroenterol 2008; 14: 6960-6969.   DOI   ScienceOn