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Prevalence of Clostridium difficile Infections in Pigs in Jeju  

Kim, Hyeong-Seok (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
Jung, Ji-Youl (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
Kang, Sang-Chul (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
Shin, Bo-Moon (Department of Laboratory Medicine, Sanggye-Paik Hospital, Inje University)
Lee, Eun-Joo (Department of Laboratory Medicine, Sanggye-Paik Hospital, Inje University)
Hwang, Eui-Kyung (College of Life Science and Natural Resources, Sangji University)
Park, Bong-Kyun (College of Veterinary Medicine, Seoul National University)
Kim, Dae-Yong (College of Veterinary Medicine, Seoul National University)
Kim, Jae-Hoon (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
Publication Information
Journal of Veterinary Clinics / v.28, no.2, 2011 , pp. 232-235 More about this Journal
Abstract
Clostridium (C) difficile has been recognized as an important emerging pathogen in both humans and animals. The prevalence of C. difficile in rectal feces and frozen colons of 132 pigs with diarrhea from the Jeju Island was investigated by polymerase chain reaction (PCR) to detect C. difficile toxin A and B genes. PCR findings revealed toxin A and B in 5 pigs (3.8%), including 2 suckling pigs, 2 weaned pigs and 1 growing pig. The result of PCR was closely matched histopathologic lesions of C. difficile in large intestines of pigs. Histopathologically, the cecum and colons of C. difficile toxin-positive pigs had severe submucosal and mesocolonic edema. Mucosal lesions ranged from random single cell necrosis and exfoliation to segmental, transmural necrosis of the cecum and colon. According to bacteriology, 4 C. difficile-positive pigs (80%) were co-infected with Salmonella typhimurium.
Keywords
Clostridium difficile; diarrhea; PCR; pig; toxin;
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1 Yaeger MJ, Kinyon JM, Songer JG. A retrospective, case control study evaluating the association between Clostridium difficile toxins in the colon of neonatal swine and gross and microscopic lesions. J Vet Diagn Invest 2007; 19: 52-59.   DOI   ScienceOn
2 Songer JG, Uzal FA. Clostridial enteric infections in pigs. J Vet Diagn Invest 2005; 17: 528-536.   DOI   ScienceOn
3 Waters EH, Orr JP, Clark EG, Schaufele CM. Typhlocolitis caused by Clostridium difficile in sucking piglets. J Vet Diagn Invest 1998; 10: 104-108   DOI   ScienceOn
4 Yaeger M, Funk N, Hoffman L. A survey of agents associated with neonatal diarrhea in Iowa swine including Clostridium difficile and porcine reproductive and respiratory syndrome virus. J Vet Diagn Invest 2002; 14: 281-287.   DOI   ScienceOn
5 Shin BM, Lee EJ. Comparison of toxin A enzyme linked fluorescence assay and latex agglutination based on Clostridium difficile culture and toxin A and B PCR assay. Korean J Clin Microbiol 2005; 8: 130-135.
6 Keel MK, Songer JG. The comparative pathology of Clostridium difficile-associated disease. Vet Pathol 2006; 43: 225-240   DOI   ScienceOn
7 Shin BM, Kuak EY. Chracterization of a toxin A-negative, toxin B-positive variant strain of Clostridium difficile. Korean J Lab Med 2006; 26: 27-31   DOI   ScienceOn
8 Songer JG. Clostridia as agents of zoonotic disease. Vet Microbiol 2010; 140: 399-404.   DOI
9 Jones MA, Hunter D. Isolation of Clostridium difficile from pigs. Vet Rec 1983; 112: 253.   DOI   ScienceOn
10 Jung K, Ha SK, Chung HK, Kim J, Cho WS, Choi C, Chae C. Archival PCR-based diagnosis of Clostridium difficile in piglets. Vet Rec 2003; 153: 466-467   DOI   ScienceOn
11 Kiss D, Bilkei G. A new periparturient disease in eastern Europe, Clostridium difficile causes postparturient sow losses. Theriogenology 2005; 63: 17-23   DOI   ScienceOn
12 Lyerly DM, Krivan HC, Wilkins TD. Clostridium difficile: its disease and toxins. Clin Microbiol Rev 1988; 1: 1-18
13 Gould LH, Limbago B. Clostridium difficile in food and domestic animals: a new foodborne pathogen? Clin Infect Dis 2010; 51: 577-582.   DOI   ScienceOn
14 Alvarez-Perez S, Blanco JL, Bouza E, Alba P, Gibert X, Maldonado J, Garcia ME. Prevalence of Clostridium difficile in diarrhoeic and non-darrhoeic piglets. Vet Microbiol 2009; 137: 302-305.   DOI   ScienceOn