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The Reduction of Hydrogen Peroxide in Viable Boar Sperm Cryopreserved in the Presence of Catalase  

Kim, Su-Hee (Department of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University)
Lee, Young-Jun (Department of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University)
Kang, Tae-Woon (Department of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University)
Kim, Yong-Jun (Department of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University)
Publication Information
Journal of Veterinary Clinics / v.28, no.1, 2011 , pp. 13-19 More about this Journal
Abstract
Semen cryopreservation induces the formation of reactive oxygen species (ROS), and the ROS cause sperm damage. We aimed to investigate the effects of the antioxidative enzyme catalase (CAT) on sperm quality and ROS during cryopreservation. Sperm rich fractions collected from five Duroc boars were cryopreserved in freezing extender with (200 or 400 U/mL) or without CAT (control). After thawing, sperm motility, viability, normal morphology, plasma membrane integrity, mitochondrial function and intracellular ROS were evaluated. CAT significantly improved total sperm motility at a concentration of 400 U/mL (P < 0.05), but didn't improve progressive sperm motility, viability, morphological defects, plasma membrane integrity and mitochondrial function in frozen-thawed boar sperm. In evaluation of ROS, CAT had no effect on reduction in ${\cdot}O_2$, but scavenged $H_2O_2$ in viable frozen-thawed boar sperm at concentrations of 200 and 400 U/mL (P < 0.05). In conclusion, CAT was not enough to improve quality of frozen-thawed sperm, but can reduce $H_2O_2$ generation in viable boar sperm during cryopreservation.
Keywords
boar sperm; catalase; cryopreservation; ROS;
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