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Time-resolved Fluoroimmunoassay (TR-FIA) Analysis of Fecal Progesterone and Estradiol in Leopard Cats (Prionailurus bengalensis)  

Kim, Young-Seob (Seoul Zoo)
Kim, Ji-Yong (College of Veterinary Medicine, Seoul National University)
Jung, So-Young (Seoul Zoo)
Lee, Bong-Joo (College of Veterinary Medicine, Chonnam National University)
Shin, Nam-Shik (College of Veterinary Medicine, Seoul National University)
Publication Information
Journal of Veterinary Clinics / v.27, no.6, 2010 , pp. 693-697 More about this Journal
Abstract
This study, conducted with four leopard cats (Prionailurus bengalensis), used time-resolved fluoroimmunoassay (TR-FIA) to analyze estradiol and progesterone concentrations in fecal samples. We measured fecal samples taken during estrus period, diestrus period, pregnancy and non-pregnancy period. During estrus (February), the mean minimum estradiol concentration was $4.02{\pm}1.9$ng/g, and the mean maximum was $86.01{\pm}35.2$ng/g (dry fecal weight). During diestrus (November), the mean minimum estradiol concentration was $4.42{\pm}1.32$ng/g and mean maximum was $15.62{\pm}6.48$ng/g (dry fecal weight). Midgestation (April), the mean minimum progesterone concentration was $427{\pm}24.49$ng/g and the mean maximum was $1490{\pm}265.27$ng/g. During non-pregnancy (November), the mean minimum progesterone concentration was $71.25{\pm}29.61$ng/g and the mean maximum was $291.75{\pm}90.30$ng/g. These results suggest that steroid hormone analysis of feces using TR-FIA is a valid method for noninvasively determining ovarian activity associated with estrus and pregnancy in leopard cats. This study will contribute to building breeding management and reproductive plans for endangered species.
Keywords
leopard cat; Prionailurus bengalensis; TR-FIA; estradiol; progesterone;
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