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IgG Humoral Immune Response to Extract Proteins of Malassezia Pachydermatis Isolated from a Dog with Atopic Dermatitis (Ad)  

Kim, Eun-Tae (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University)
Kim, Ha-Jung (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University)
Lim, Chae-Young (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University)
Park, Hee-Myung (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University)
Publication Information
Journal of Veterinary Clinics / v.25, no.5, 2008 , pp. 340-345 More about this Journal
Abstract
Malassezia pachydermatis (M. pachydermatis) is a component of the normal cutaneous flora of the dog and atopic dermatitis (AD) is one of the most common diseases associated with Malassezia overgrowth in dogs. The purpose of this study was to investigate the humoral response (IgG) to extracts of M. pachydermatis of in a dog with AD. We used Western immunoblotting to identify allergens of M. pachydermatis. Gel electrophoresis of extracts proteins and immunoblotting of sera samples in both an atopic dog and a non-atopic dog were compared. Proteins of 18, 21, 26, 32, 34, 38, 40, 42, 46, 58, 64, 75, 85, and 120 kDa were observed in a serum of atopic dog. However, when serum of a non-atopic dog was used, protein bands were not identified except binding in 50 kDa protein. The results of this study indicate that atopic dogs with M. pachydermatis dermatitis may induce IgG response and also suggest that humoral response to M. pachydermatis could be important in pathogenesis of AD in dogs. However, further studies are required to identify roles of humoral response to M. pachydermatis in canine AD.
Keywords
atopic dermatitis; dog; IgG; humoral response; Malassezia pachydermatis;
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1 Zargari A, Harfast B, Johansson S, Johansson SG, Scheynius A. Identification of allergen components of the opportunistic yeast Pityrosporum orbiculare by monoclonal antibodies. Allergy 1994; 49: 50-56   DOI   ScienceOn
2 Rowntree S, Cogswell JJ, Platts-Mills TA, Mitchell EB. Development of IgE and IgG antibodies to food and inhalant allergens in children at risk of allergic disease. Arch Dis Child 1985; 60: 727-735   DOI
3 Bond R, Elwood CM, Littler RM, Pinter L, Lloyd DH. Humoral and cell-mediated responses to Malassezia pachydermatis in healthy dogs and dogs with Malassezia dermatitis. Vet Rec 1998; 143: 381-384   DOI   ScienceOn
4 Charach M. Malassezia dermatitis. Can vet J 1997; 38: 311-314
5 Fraser MA, McNeil PE, Gettinby G. Examination of serum total IgG1 concentration in atopic and non-atopic dogs. J Small Anim Pract 2004; 45: 186-190   DOI   ScienceOn
6 Halliwell RE, Longino SJ. IgE and IgG antibodies to flea antigen in differing dog populations. Vet Immunol Immunopathol 1985; 8: 215-223   DOI   ScienceOn
7 Hill PB, Moriello KA, DeBoer DJ. Concentrations of total serum IgE, IgA, and IgG in atopic and parasitized dogs. Vet Immunol Immunopathol 1995; 44: 105-113   DOI   ScienceOn
8 Morales, CA, Schultz KT, DeBoer DJ. Antistaphylococcal antibodies in dogs with recurrent staphylococcal pyoderma. Vet Immunol Immunopathol 1994; 42: 137-147   DOI   ScienceOn
9 Nuttall TJ, Halliwell RE. Serum antibodies to Malassezia yeasts in canine atopic dermatitis. Vet Dermatol 2001; 12: 327-332   DOI   ScienceOn
10 Rasool O, Zargari A, Almqvist J, Eshaghi H, Whitley P, Scheynius A. Cloning, characterization and expression of complete coding sequences of three IgE binding Malassezia furfur allergens, Mal f 7, Mal f 8 and Mal f 9. Eur J Biochem 2000; 267: 4355-4361   DOI   ScienceOn
11 Jensen-Jarolim EP, Poulsen LK, With H, Kieffer M, Ottevanger V, Stahl SP. Atopic dermatitis of the face, scalp, and neck: type | reaction to reaction to the yeast Pityrosporum ovale? J Allergy Clin Immunol 1992; 89: 44-51   DOI
12 Bond R, Ferguson EA, Curtis CF, Craig JM, Lloyd DH. Factors associated with elevated cutaneous Malassezia pachydermatis populations in dogs with pruritic skin disease. J Small Anim Pract 1996; 37: 103-107   DOI   ScienceOn
13 Chen TA, Halliwell REW, Hill PB. IgG responses to Malassezia pachydermatis antigens in atopic and normal dogs. Veterinary dermatology 11 (Suppl 1), 13   DOI   ScienceOn
14 Jenmalm MC, Bjorksten B. Development of immunoglobulin G subclass antibodies to ovalbumin, birch and cat during the first eight years of life in atopic and non-atopic children. Pediatr Allergy Immunol 1999; 10: 112-121   DOI   ScienceOn
15 Hites MJ, Kleinbeck ML, Loker JL, Lee KW. Effect of immuno-therapy on the serum concentrations of allergenspecific IgG antibodies in dog sera. Vet Immunol Immunopathol 1989; 22: 39-51   DOI   ScienceOn
16 Bond R, Lloyd DH. Immunoglobulin G responses to Malassezia pachydermatis in healthy dogs and dogs with Malassezia dermatitis. Vet Rec 2002; 150: 509-512   DOI   ScienceOn
17 Chen TA, Halliwell REW, Pemberton AD, Hill PB. Identification of major allergens of Malassezia pachydermatis in dogs with atopic dermatitis and Malassezia overgrowth. Vet Dermatol 2002; 13: 141-150   DOI   ScienceOn
18 Holt PG., McMenamin C. Defence against allergic sensitization in the healthy lung: the role of inhalation tolerance. Clin Exp Allergy 1989; 19: 255-262   DOI   ScienceOn
19 Mauldin EA, Scott DW, Miller JWH, Smith CA. Malassezia dermatitis in the dog: a retrospective histopathological and immunopathological study of 86 cases (1990-95). Vet Dermatol 1997; 8: 191-202   DOI   ScienceOn
20 Scott DW, Miller WH, Griffin CE. Canine atopic dermatitis. In: Muller and Kirk's Small Animal Dermatology, 6th edn. Philadelphia: W. B. Saunders 2001: 363-374
21 Yasueda H, Hashida-Okado T, Saito A, UchidaK, Kuroda M, Onishi Y, Takahashi K, Yamaguchi H, Takesako K, Akiyama K. Identification and cloning of two novel allergens from the lipophilic yeast, Malassezia furfur. Biochem Biophys Res Commun 1998; 248: 240-244   DOI   ScienceOn
22 Willemse T. Atopic skin disease: a review and reconsideration of diagnostic criteria. J. Small. Anim. Pract 1986; 27: 771-778   DOI
23 Mason KV. Malassezia dermatitis and otitis. In: Current Veterinary Therapy. Philadelphia: W. B. Saunders. 1992: 544-546
24 Habibah A, Catchpole B, Bond R. Canine serum immunoreactivity to M. pachydermatis in vitro is influenced by the phase of yeast growth. Vet dermatol 2005; 16: 147-152   DOI   ScienceOn
25 Morris DO, Clayton DJ, Dorobatz KJ, Felsburg PJ. Response to Malassezia pachydermatis by peripheral blood mononuclear cells from clinically normal and atopic dogs. Am J Vet Res 2002; 63; 358-362   DOI   ScienceOn
26 Lindborg M, Magnusson CG, Zargari A, Schmidt M, Scheynius A, Crameri R, Whitley P. Selective cloning of allergens from the skin colonizing yeast Malassezia furfur by phage surface display technology. J Invest Dermatol 1999; 113: 156-161   DOI   ScienceOn
27 Lintu, P., Savolainen, J., Kalimo, K. IgE antibodies to protein and mannan antigens of pityrosporum ovale in atopic dermatitis patients. Clin Exp Allergy 1997; 27: 87-95   DOI   ScienceOn
28 Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-685   DOI   ScienceOn
29 Onishi Y, Kuroda M, Yasueda H, Akemi S, Eiko SK, Susumu T, Takashi HO, Tomoko Y, Katsuhisa U, Hideyo Y, Kazuo A, Ikunoshin K, Kazutoh T. Two-dimensional electrophoresis of Malassezia allergens for atopic dermatitis and isolation of Mal f 4 homologous with mitochondrial malate dehydrogenase. Eur J Biochem 1999; 261: 148-154   DOI   ScienceOn
30 Scott DW, Miller WH, Griffin CE. Canine atopic dermatitis. In: Muller and Kirk's Small Animal Dermatology, 6th edn. Philadelphia: W. B. Saunders 2001: 574-601
31 Schmidt M, Zargari A, Holt P, Lindbom L, Hellman U, Whitley P, van der Ploeg I, Harfast B, Scheynius A. The complete cDNA sequence and expression of the first major allergenic protein of Malassezia furfur, Mal f 1. Eur J Biochem 1997; 246: 181-185   DOI   ScienceOn
32 Bond R, Sant RE. The recovery of Malassezia pachydermatis from canine skin. Vet Dermatol Newsletter 1993; 15: 25-27