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Clinical Application of PCR-RFLP for the Differentiation of Trichophyton mentagrophytes var. erinacei in the Facial Dermatitis of Household African Pygmy Hedgehog (Erinaceus albiventris)  

Han, Jae-Ik (College of Veterinary Medicine, Chungbuk National University)
Na, Ki-Jeong (College of Veterinary Medicine, Chungbuk National University)
Publication Information
Journal of Veterinary Clinics / v.25, no.3, 2008 , pp. 211-214 More about this Journal
Abstract
This report describes a case of severe and prolonged dermatophytosis in a hedgehog that was diagnosed by PCR-RFLP, a rapid and usefulness technique for identification of many causative agents and hereditary characters. A 5-month-old female hedgehog was presented with grade 2 facial pruritus, scaling, encrustation and hemorrhage. Cytology of exudates on the face showed a suspected fungal infection. A culture and tape imprint test of the cultured colony showed many hyphae and microcornidia, suspected to belong to the Trichophyton species. In the PCR-RFLP with MvaI and Hinf I, Trichophyton mentagrophytes var. erinacei was finally identified as a causative agent. The patient completely recovered after application of nystatin cream for 17 days.
Keywords
African pygmy hedgehog; Trichophyton mentagrophtes; PCR-RFLP;
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