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A Real Time PCR Assay for Detection and Quantitation of Canine Parvovirus Type 2 in the Feces of Dogs with Parvovirus Infection  

koh Min-Soo (College of Veterinary Medicine, Chonnam National University)
Sin So-Yeon (College of Veterinary Medicine, Chonnam National University)
Kim Yong-Hwan (Gwangju Metropolitan Health & Environment Research Institute)
Koh Ba-Ra-Da (Gwangju Metropolitan Health & Environment Research Institute)
Lee Bong-Joo (College of Veterinary Medicine, Chonnam National University)
Publication Information
Journal of Veterinary Clinics / v.22, no.4, 2005 , pp. 348-352 More about this Journal
Abstract
We described a rapid, sensitive and reproducible real-time PCR assay for detection and quantitation of canine parvovirus type 2 in the feces of dogs with parvovirus infection. The method was demonstrated to be highly specific and sensitive, allowing a precise canine parvovirus type-2 quantitation over range of eight orders of magnitude from $10^2\;to\;10^9$ copies of standard DNA. Then, fecal samples from parvovirus infected dogs were analyzed by conventional PCR and real-time PCR. Real-time PCR is more sensitive than conventional PCR, allowing to detect low viral titers of CPV-2 in infected dogs. By real-time PCR, a wide range of parvovirus particles was found in the samples from $1.45\times10^6\;to\;9.45\times10^8$ copies/0.01g of feces. However, when dogs are in infection of parvovirus, it is difficult to prove that the numbers of peripheral blood leukocytes are correlated with those of fecal shedding virus.
Keywords
canine parvovirus; real-time PCR;
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