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Immunoenhancing Effect of Conjugated Linoleic Acids on Phagocytic Activity of Porcine Peripheral Blood Phagocytes  

Kang Ji-houn (충북대학교 수의과대학 수의학과 및 동물의학연구소)
Kim Ju-hyang (충북대학교 수의과대학 수의학과 및 동물의학연구소)
Chung Chung-soo (충북대학교 농과대학 축산학과)
Lee Chul-young (진주산업대학교 국제축산개발학과)
Yang Mhan-pyo (충북대학교 수의과대학 수의학과 및 동물의학연구소)
Publication Information
Journal of Veterinary Clinics / v.21, no.4, 2004 , pp. 336-342 More about this Journal
Abstract
The immunoenhancing effect of CLA isomers (CLA mixture, 10t-12c CLA, 9c-11c CLA, 9c-11c CLA, and 9t-11t CLA) on phagocytic activity of porcine peripheral blood leukocytes was examined. The phagocytic activities of peripheral blood mononuclear cells (PBMC) and polymorphonuclear cells (PMN) were analyzed by a flow cytometry system. The direct treatments of CLA isomers have no effect on phagocytosis of PMN as well as PBMC composed of approximately 10% monocytes and 90% lymphocytes. However, the phagocytic activities of PMN and monocyterich fraction from PBMC were remarkably enhanced by culture supernatant from PBMC treated with CLA mixture, 10t-12c CLA and 9c-11t CLA but not 9c-11c CLA and 9t-11t CLA. The phagocytic activity of PBMC was not enhanced by culture supernatant from PBMC treated with all CLA isomers. These results indicated that CLA isomers such as CLA mixture, l0t -12c CLA and 9c-11t CLA have an enhancing effect on phagocytosis of PMN and monocytes, which may be mediated through active humoral substances produced by CLA-stimulated PBMC. This study suggested that CLA stimulates PBMC to elaborate soluble factor(s), which may be an important mechanism for the enhancement of phagocytosis in non-specific immunity.
Keywords
pig; conjugated linoleic acid; peripheral blood phagocytes; phagocytosis;
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