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Bone Regeneration Effects of Copper Oxide on Fibular Fracture in Rabbits  

정윤정 (강원대학교 수의학과)
이창훈 (원자력병원, 세포생물학실험실)
배일주 (천지산 연구소(주))
남일 (프라마스(주))
정성목 (서울대학교 수의과대학)
남치주 (서울대학교 수의과대학)
서강문 (서울대학교 수의과대학)
Publication Information
Journal of Veterinary Clinics / v.20, no.4, 2003 , pp. 458-466 More about this Journal
Abstract
The bone regeneration effects of copper oxide on experimentally induced fibular fracture were examined in 36 New Zealand white male rabbits. They were divided into two groups: non-treated group (control group) and copper oxide treated group (treatment group). A fibular fracture was created by an osteotomy in the middle of the fibula and 62.5 mg/kg of copper oxide was orally administrated during 7 days after operation in the treatment group. Radiological findings, histopathological examinations and hematoserological findings were observed to evaluate the bone regeneration effects of copper oxide on fibula fracture during 9 weeks. In radiological findings, the area of bone regeneration at the fracture site of the treatment group was significantly wider from 3 weeks to 6 weeks after administration of copper oxide than those of the control group (p < 0.05). In histopathological examinations, fracture healing in treatment group was faster than in control group. Also, histopathological responses of thick bony trabeculae and new bone marrow formation were shown in the treatment group, whereas many fibrous tissues and cartilages were mainly observed in the control group. No specific effects of copper oxide on the body was found in hematological and serological test during experimental period. These results showed that the copper oxide had a potential therapeutic application in the treatment of fracture and bone trauma.
Keywords
copper oxide; bone regeneration; fibular fracture; rabbit;
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1 Banno S, Niita M, Kikuchi M, Wakita A, Takada K, Mitomo Y, Niimi T, Yamamoto T. Anemia and neutropenia in elderly patients caused by copper deficiency for long-term enteral nutrition. Rinsho Ketsueki 1994; 35: 1276-1281
2 Behr W, Bamert J. Quantification of bone alkaline phos-phatase in semm by precipitation with wheat-germ lectin: a simplified method and its clinical plausibility. Clin Chem 1986; 32: 1960-1966
3 Fini M, Aldini NN, Cane V, Zaffe D, Giavaresi G, Rocca M, Guzzardella GA, Giardino R. Effects of essential amino acids and lactose on bony fractures and defects in rabbits: a preliminary histomorphometiic study. Arch Orthop Trauma Surg 1999; 119: 39-45   DOI   ScienceOn
4 Fleming CR, Hodges RE, Hurley LS. A prospective study of serum copper and zinc levels in patients recieving total parenteral nutrition. Am J Clin Nutr 1976: 29: 70
5 Guo F, Wang T, Chen A, Sun S, Li Z. Ovine calcined bone for the repair of radial defect in a rabbit inodel. Tongji Med. Univ 2000; 20: 242-245   DOI   ScienceOn
6 Kato T, Kawaguchi H, Hanada K, Aoyama I, Hiyama Y, Nakamura T, Kuzutani K, Tamura M, Kurokawa T, Nakamura K. Single local injection of recombinant fibroblast growth factor-2 stimulates healing of segmental bone defects in rabbits. J Orthop Res 1998; 16: 654-659   DOI   ScienceOn
7 Opsahl W, Zeronian H, Ellison M, Lewis D, Rucker RB, Riggins RS. Role of copper in collagen cross-linking and its influence on selected mechanical properties of chick bone and tendon. J Nutr 1982; 112: 708-716
8 Rasmussen H. Bordier P. The physiological and cellular basis of metabolic bone disease. Baltimore: Williams & Wilkins 1974:81
9 Rico H, Gomez-Raso N, Revilla M, Hemandez ER, Seco C, Paez E, Crespo E. Effects on bone loss of manganese alone or with copper supplement in ovariectomized rats. A morpho-metric and densitomeric study. Eur J Obstet Gynecol Reprod Biol 2000; 90: 97-101   DOI   ScienceOn
10 Roermund PM, Hoekstra A, Visser WJ, Renooij W. Effect of direct current stimulation on bone growth after distraction epiphysiolysis of the rabbit tibia. J Orthop Res 1990; 8: 769-775   DOI
11 Carnes WH. Role of copper in connective tissue metabolism. Fed Froc 1971; 30: 995-1000
12 Pienkowski D, Pollack SR, Brighton CT, Ghath NJ. Low-power electromagnetic stimulation of osteotomized rabbit fibulae. A randomized, blinded study. J Bone Joint Surg Am 1994; 76: 489-501
13 O'Dell B.L. Biochemistry of copper. Med. Clin. North Am.1976; 60: 687
14 Opsahl W, Zeronian H, Ellison M, Lewis D, Rucker RB, Riggins RS. Role of copper in collagen cross-linking and its influence on selected mechanical properties of chick bone and tendon. J Nutr 1982; 112: 708-716
15 Wiss DA, Ledesma-Medina J. Skeletal changes in copper deficiency following prolonged total parenteral nutrition. Orthopedics 1980; 3: 969
16 Heresi G, Castillo-Duran C, Munoz C, Arevalo M, Schlesinger L. Phagocytosis and immunoglobulins level in hypocupremic infants. Nutr Res 1985; 33: 1112-1118
17 Goodman JR, Dalman PR. Role of copper in iron localization in developing erythrocytes. Blood 1969; 34: 747
18 Milachowski K, Moschinski D, Stawinoga B. The influence of the trace-elements copper and zinc on bone healing in rabbits. Unfallheilkunde 1981, 84: 168-174
19 Tanaka Y, Hatano S, Nishi Y, Usui T. Nutritional copper deficiency in Japenese infant on formula. J Pediatr 1980; 96: 255-257   DOI
20 Staley GP, van der Lugt JJ, Axsel G, Loock AH. Congenital skeletal malformation in Holstein calves associated with putative manganese deficiency. J S Afr Vet Assoc 1994; 65: 73-78
21 O'Dell BL, Smith RM, King RA. Brain catecholamines and copper deficiency in lambs. Fed Proc 1974; 33; 668
22 Piez KA. Cross-linking of collagen and elastin. Annu Rev Biochem 1968; 37: 547   DOI   ScienceOn
23 Rico H, Roca-Botran C, Hemandez ER, Seco C, Paez E, Valencia MJ, Villa LF. The effect of supplemental copper onosteopenia induced by ovariectomy in rats. Menopause 2000; 7: 413-416   DOI   ScienceOn
24 Heller RM, Kirchner SG, O'Neill JA, Hough AJ, Howard L, Kramer SS, Green HL. Skeletal changes of copper deficiency in infants receiving prolonged total parenteral nuthtion. J Pediatr 1978; 92: 947-949   DOI
25 Wilson T, Katz JM, Gray DH. Inhibition of active bone resorption by copper. Cal Tissue Int 1981; 33: 35-39   DOI   ScienceOn
26 Rosalki SB, Foo AY. Two new methods for separating and quantifying bone and liver alkaline phosphatase isoenzymes in plasma. Clin Chem 1984; 30: 1182-1186
27 Dollwet HH, Sorenson JR. Roles of copper in bone main-tenance and healing.. Biol. Trace Elem Res 1988; 18: 39-48   DOI   ScienceOn
28 Hart EB, Steenbock H, Waddell J, Elvehjem CA. Iron nutrition. VII. Copper is a supplement to iron for hemoglobin building in the rat. J Biol Chem 1928; 77: 797-812
29 Underwood EJ. Trace elements in human and animal nutrition, 4th ed. New York: Academic Press. 1977: 185
30 Rucker RB, Tinker D. Structure and metabolism of arterial elastin. Int Rev Exp Patho. 1977; 17: 1-47
31 Haas EM. www.natural-healing.co.kr/sup/mineral-copper.htm. 2001
32 Danks DM. Copper deficiency in human. Annu Rev Nutr 1988; 8: 235-257   DOI   ScienceOn
33 Stausak D, Mercer JF, Dieter HH, Strmmel W, Multhaup S. Copper in disorders with neurological symptoms: Alzheimer's Menkes, and Wilson disease. Brain Res Bull 2001; 55: 175-185   DOI   ScienceOn
34 Rucker RB, Rucker BR, Mitchell AE, Chang TC, Clegg M, Kosonen T, Uriu-Adams JY, Tchapahan EH, Fishman M, Keen CL. Activation of chick tendon lysyl oxidase in response to dietary copper. J Nutr 1999; 129; 2143-2146
35 Okano T. Effects of essential trace elements on bone turnover-in relation to the osteoporosis. Nippon Rinsho 1996; 54: 148-154
36 Yilmaz N, Ozaslan J. Biochemical bone tumover markers in patients with ankylosing spondylitis. Clin Rheumatol 2000; 19: 92-98   DOI   ScienceOn
37 Baxter JH. Bone disorder in copper deRcient puppies. Am J Physiol 1951; 167: 766
38 Takahashi S, Sugimoto M, Kotoura Y, Nishimatsu H, Shibamoto Y, Abe M, Yamamuro T. The effects of intraoperative radiotherapy on bone-healing ability in relation to different doses and postradiotherapy intervals. Int J Radiat Oncol Biol Phys 1994; 30: 1147-1152   DOI   ScienceOn
39 Allen TM, Manoli A 2nd, LaMont RL. Skeletal changes associated with copper deficiency. Clin Orthop 1982; 168: 206-210
40 Suttle NF, Angus KW, Nisbet DI, Field AC. Osteoporosis in copper depleted lambs. J Com Path 1972; 82: 93-97   DOI   ScienceOn