Browse > Article

Possible Process of Safflower Seed on New Bone formation by 2-Dimensional Electrophoresis  

라도경 (경상대학교 수의과대학(동물의학연구소))
정태성 (경상대학교 수의과대학(동물의학연구소))
김종수 (경상대학교 수의과대학(동물의학연구소))
송해룡 (경상대학교 의과대학)
김용환 (경상대학교 수의과대학(동물의학연구소))
강정부 (경상대학교 수의과대학(동물의학연구소))
강호조 (경상대학교 수의과대학(동물의학연구소))
연성찬 (경상대학교 수의과대학(동물의학연구소))
신기욱 (경상대학교 수의과대학(동물의학연구소))
Publication Information
Journal of Veterinary Clinics / v.19, no.1, 2002 , pp. 49-54 More about this Journal
Abstract
Korean safflower (Carthami Flos) seed has been known to have healing effects on both bone fracture and osteoporosis. On the base of such a notice, this experiment was carried out to explore the effects of safflower seed on bone formation and bone repair. In addition, the healing mechanism was evaluated by analysing serum after feeding the seed to experimental. animals. The effect of Korean safflower seed were evaluated with 40 rats,3-month old. Forty Sprague-Dawley rats composed of 20 male and 20 female were underwent unilateral tibial defect and then fastened with unilateral fixators. The operated rats were divided into two groups depending on the composition of diet, such as positive control group fed normal diet (C-OP group) and safflower seed group fed 30% of safflower seed diet and 70% of normal diet (S-OP group). Postoperative radiographys were taken once in 2 weeks to evaluate callus formation for operated groups. In addition, a possible protein spots involved in bone recovery were examined using 2-Dimensional Gel Electrophoresis (2-DE). The comparison of the radiography between C-OP and S-OP group were showed that the safflower seed diet appeared to stimulate the formation of callus in the rat. On the images of 2-BE, it was able to identify possible five protein spots, having pl from 4 to 5 and molecular weight range from 24 to 26 kDa, involved in bone formation and repair, since no differing protein spots were found the two between groups except the five spots. No differences were observed between two groups before operation, but clear and bigger protein spots were observed from the S-OP group compared with C-OP group on 6 and 9 weeks post operation. These protein spots were, however, showed similar sizes and densities between two groups in 12 weeks later. The transformation of protein spots was suggested that these protein spots were involved in bone formation and recovery, in addition safflower seed might induce the formation of factors and activate these factors. In conclusion, this study suggest that safflower seed influence a variety of factors in the course of bone formation or the periods of remedy.
Keywords
safflower seed; fracture; Dimensional Gel Electrophoresis; images; osteoporosis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 The role of Biochemical tests in the screening and monitoring of osteoporosis /
[ Price, C.P.;Thomson P.W. ] / Ann. Clin. Biochem
2 A rapid method for determing the oil concent o Safflower and sunflower seeds /
[ Kennedy, W.K.;Unrau, J. ] / Agron. J.   DOI
3 Studies in Indian Oil Seed. No.3 Carthamus tinctorious L. The types of Safflower /
[ Khan, A.R. ] / Memoris. Dept. Agri. India. Bot. Ser.
4 Proteomics in reproductive research: the potential importance of proteomics to research in reproduction /
[ Brewis, I. A. ] / Hum.Reprod   DOI   ScienceOn
5 Proteomics in human disease: cancer, heart and infectious diseases /
[ Jungblut, P. R. ] / Electrophoresis   DOI   ScienceOn
6 Proteomics: quantitative and physical mapping of cellular proteins /
[ Blackstock, W. P.;M, P, Weir ] / Trends Biotechnol   DOI   ScienceOn
7 /
[ Kee Chang Huang ] / The Pharmacology of Chinese Herbs
8 Bone protective effects of phytoestrogens extracted from safflower seeds in ovariectomized rats and osteoblast cells /
[ Kim, H.J.(et al.) ] / 기초의학회 학술대회
9 /
[ Banks W.J. ] / Applied veterinary histology(2nd ed.)
10 A dynamic two-dimensional polyacrylamide gel electrophoresis database: the mycobacterial proteome via Internet /
[ Mollenkopf, H. J.(et al.) ] / Electrophoresis   DOI   ScienceOn
11 Quantitative proteomics [news] /
[ Mann, M. ] / Nat.Biotechnol   DOI   ScienceOn
12 /
[ 강호석 ] / 조직학(제 2판)
13 Advances in protein solubilisation for two-dimensional electrophoresis /
[ Herbert, B ] / Electrophoresis   DOI   ScienceOn
14 /
[ Link, A.J.(et al.) ] / 2D Proteome Analysis Protocol in Methods in Mol. Biol.
15 Safflower /
[ Beech, D.F. ] / Fiend Crop. Astr
16 Effects of fertilization and papulation rate-spacing on Safflower yield and other characteristics /
[ Nast, H. G.(et al.) ] / Agron. J.
17 /
[ 山口一孝 ] / 식물성분분석법(상)
18 /
[ 이창복 ] / 대한식물도감
19 /
[ 안덕균;육창수 ] / 현대본초학
20 東醫寶鑑5 /
[ 허준 ] / 12.풀부
21 Proteome Databases /
[ Bairoch, A.;M. R. Wilkins(ed.);K. L. Williams(ed.);R. D. Appl;D. F. Hochstrasser(ed.) ] / Proteome Research: New Frontiers in Functional Genomics
22 Proteomics: quantitative and physical mapping of cellular proteins /
[ Lackstock, W. P.;Weir, M. P. ] / Trends Biotechnol   DOI   ScienceOn
23 A comparative study of mouse liver proteins arylated by reactive metabolites of acetaminophen and its nonhepatotoxic regioisomer, 3'-hydroxyacetanilide /
[ Myers, T. G.(et al.) ] / Chem.Res.Toxicol
24 Two-dimensional gel electrophoresis and immunoblotting of human serum albumin modified by reaction with penicillins /
[ Marzocchi, B.(et al.) ] / Electrophoresis   DOI   ScienceOn
25 A proteome analysis of livers from obese (ob/ob) mice treated with the peroxisome proliferator WY14,643 /
[ Edvardsson, U. M.(et al.) ] / Electrophoresis   DOI   ScienceOn