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http://dx.doi.org/10.3349/ymj.2018.59.9.1079

Effects of Antioxidant Tempol on Systematic Inflammation and Endothelial Apoptosis in Emphysematous Rats Exposed to Intermittent Hypoxia  

Zhao, Haiyan (Respiratory Department of Tianjin Medical University General Hospital)
Zhao, Yaping (Respiratory Department of Tianjin Medical University General Hospital Airport Hospital)
Li, Xin (Respiratory Department of Tianjin Medical University General Hospital)
Xu, Leiqian (Respiratory Department of Tianjin Medical University General Hospital)
Jiang, Fangxin (Department of Life Sciences, State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University)
Hou, Wanju (Respiratory Department of Tianjin Medical University General Hospital)
Dong, Lixia (Respiratory Department of Tianjin Medical University General Hospital)
Cao, Jie (Respiratory Department of Tianjin Medical University General Hospital)
Publication Information
Yonsei Medical Journal / v.59, no.9, 2018 , pp. 1079-1087 More about this Journal
Abstract
Purpose: Obstructive sleep apnea and chronic obstructive pulmonary disease are independent risk factors of cardiovascular disease (CVD), and their coexistence is known as overlap syndrome (OS). Endothelial dysfunction is the initial stage of CVD; however, underlying mechanisms linking OS and CVD are not well understood. The aim of this study was to explore whether OS can lead to more severe inflammation and endothelial apoptosis by promoting endothelial dysfunction, and to assess the intervention effects of antioxidant tempol. Materials and Methods: Male Wistar rats (n=66) were exposed to normal oxygen [normal control (NC) group], intermittent hypoxia (IH group), cigarette smoke (CH group), as well as cigarette smoke and IH (OS group). Tempol intervention was assessed in OS group treated with tempol (OST group) or NaCl (OSN group). After an 8-week challenge, lung tissues, serum, and fresh blood were harvested for analysis of endothelial markers and apoptosis. Results: The levels of intracellular adhesion molecule-1, vascular cellular adhesion molecule-1, and apoptosis in circulating epithelial cells were the highest in OS group and the lowest in NC group. These levels were all greater in IH group than in CH group, and were lower in OST group than in OS and OSN groups (all p<0.001). Conclusion: Synergistic effects of IH with cigarette smoke-induced emphysema produce a greater inflammatory status and endothelial apoptosis. OS-related inflammation and endothelial cell apoptosis may play important roles in promoting cardiovascular dysfunction, and antioxidant tempol could achieve a partial protective effect.
Keywords
Overlap syndrome; intermittent hypoxia; emphysema; inflammation; endothelial dysfunction; antioxidant;
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