Browse > Article
http://dx.doi.org/10.15616/BSL.2021.27.4.334

Effects of Inhibiting Glycoprotein MUC5AC by Seaweed Ecklonia cava Extract in human Airway Epithelial Cells  

Lee, Sung-Gyu (Department of Medical Laboratory Science, College of Health Science, Dankook University)
Kwon, Sang-Oh (Department of Medical Laboratory Science, College of Health Science, Dankook University)
Publication Information
Biomedical Science Letters / v.27, no.4, 2021 , pp. 334-339 More about this Journal
Abstract
In the present study, antioxidant and MUC5AC mucin inhibition activities were measured in Ecklonia cava (E. cava) extract. The E. cava extract showed the total polyphenol and flavonoid contents of 607.40±19.44 ㎍ GAE/mg and 13.33±5.28 ㎍ QE/mg, respectively. The free radical scavenging activity of E. cava extract was high in the DPPH radical scavenging activity (RC50 7.08 ㎍/mL) and ABTS+ radical scavenging activity (RC50 4.74 ㎍/mL). Also, we investigated whether E. cava extract affects airway MUC5AC mucin gene expression, production and secretion induced by phorbol 12-myristate 13-acetate (PMA) from NCI-H292 cells. Cells were treated with E. cava extract and then stimulated with PMA for 24 h. The E. cava extract inhibited the gene expression of MUC5AC mucin from NCI-H292 cells. This result suggests that E. cava extract can inhibit the gene expression of mucin induced by PMA through directly acting on airway epithelial cells.
Keywords
Ecklonia cava; MUC5AC; NCI-H292; PMA; Seaweed;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Ahn MJ, Yoon KD, Min SY, Lee JS, Kim JH, Kim TG, Kim SH, Kim NG, Huh H, Kim J. Inhibition of HIV-1 reverse transcriptase and protease by phlorotannins from the brown alga Ecklonia cava. Biol Pharm Bull. 2004. 27: 544-547.   DOI
2 Athukorala Y, Jung WK, Vasanthan T, Jeon YJ. Anticoagulative polysaccharide froman enzymatic hydrolysate of Ecklonia cava, Carbohydr Polym. 2006. 66: 184-191.   DOI
3 Cragg GM, Newman DJ, Weiss RB. Coral reefs, forests, and thermal vents: the worldwide exploration of nature for novel antitumor agents. Semin Oncol. 1997. 24: 156-163.
4 Dhargalkar VK, Verlecar XN. Southern Ocean seaweeds: A resource for exploration in food and drugs. Aquaculture. 2009. 287: 287-229.
5 Ellis EF. Asthma in childhood. J Allergy Clin Immunol.1985. 72: 526-539.   DOI
6 Freeman BA, Crapo JD. Biology of disease: free radicals and tissue injury. Lab Invest. 1982. 47: 412-426.
7 Kang KA, Lee KH, Park JW, Lee NH, Na HK, Surh YJ, You HJ, Chung MH, Hyun JW, Triphlorethol-A induces heme oxygenase-1 via activation of ERK and NF-E2 related factor 2 transcription factor. FEBS Lett. 2007. 581: 2000-2008.   DOI
8 Kong CS, Kim JA, Yoon NY, and Kin S. Induction of apoptosis by phloroglucinol derivative from Ecklonia cava in MCF-7 human breast cancer cells. Food Chem Toxicol. 2009. 47: 1653-1658.   DOI
9 Lillehoj EP, Kato K, Lu W, Kim KC. Cellular and Molecular Biology of Airway Mucins, Int Rev Cell Mol Biol. 2013. 139-202.
10 Re R, Pellegrini N, Proteggente A, et al. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999. 26: 1231-1237.   DOI
11 Lee SI. The level of antioxidant enzymes in red blood cells of patients with chronic obstructive pulmonary disease. Tuberc Respir Dis. 1997. 44: 104-113.   DOI
12 Kang KA, Lee KH, Chae S, Koh YS, Yoo BS, Kim JH, Ham YM, Baik JS, Lee NH, Hyun JW. Triphlorethol-A from Ecklonia cava protects V79-4 lung fibroblast against hydrogen peroxide induced cell damage. Free Radic Res. 2005. 39: 83-892.
13 Yoo CY, Kim SY, Park JW, Sung SA, Kim DA, Pakr JH, Xuan SH, Park SN. Antioxidant and Cellular Protective Activities of Ecklonia cava extracts against Reactive Oxyegen Species. J Soc Cosmet Sci Korea. 2015. 41: 287-294.   DOI
14 Folin O, Denis W. On phosphotungstic-phosphomolybdic compounds as color reagents. J Biol Chem. 1912. 12: 239-243.   DOI
15 Freeman BA, Topolosky MK, Crapo JD. Hyperoxia increases oxygen radical production in rat lung homogenates. Arch Biochem Biophys. 1982. 216: 477-484.   DOI
16 Heo SJ, Park EJ, Lee K, Jeon YJ. Antioxidant activities of enzymatic extracts from brown seaweeds. Biores Technol. 2005. 96: 1613-1623.   DOI
17 Hewson CA, Edbrooke MR, Johnston SL. PMA induces the MUC5AC respiratory mucin in human bronchial epithelial cells, via PKC, EGF/TGF-alpha, Ras/Raf, MEK, ERK and Sp1-dependent mechanisms. J Mol Biol. 2004. 344: 683-695.   DOI
18 Kim JH, Kang HM, Lee SH, Lee JY, Park LY. Antioxidant and α-glucosidase inhibition activity of seaweed extracts. Korean J Food Preserv. 2015. 22: 290-296.   DOI
19 Kim KN, Lee KW, Song CB, Jeon YJ. Cytotoxic activity of green and brown seaweeds collected from Jeju Island against four tumor cell lines. J Food Sci Nutr. 2006. 11: 17-24.
20 Lee GW, Cho YH. Antioxidant Activity of Leaf Extract from Annona muricata. Review of Korea Contents Association. 2016. 14: 43-46.
21 Mutschler E, Derendorf H. Drug actions. CRC press, Inc., Boca Raton, Florida. 1995. 410.
22 Rogers DF, Barnes PJ. Treatment of airway mucus hypersecretion. Ann Med. 2006. 38: 116-125.   DOI
23 Nieva Moreno MI, Isla MI, Sampietro AR, et al. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol. 2000. 71: 109-114.   DOI
24 Heo SJ, Park PJ. Park EJ, Cho SK, Kin SK, Jeon YJ. Antioxidative effect of proteolytic hydrolysates from Ecklonia cava on radical scavenging using ESR and H2O2-induced DNA damage. Food Sci Biotechnol. 2005. 14: 614-620.