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http://dx.doi.org/10.15616/BSL.2021.27.4.329

CD8-dependent Tumor Growth Inhibition by Tumor Cells Genetically Modified with 4-1BBL  

Kim, Hong Sung (Department of Biomedical Laboratory Science, Korea Nazarene University)
Publication Information
Biomedical Science Letters / v.27, no.4, 2021 , pp. 329-333 More about this Journal
Abstract
We previously identified that tumor cells genetically modified with a 4-1BBL co-stimulatory molecule had anticancer effects in a CT26 mouse colorectal tumor model. To identify the distinction between immune cells in a mouse tumor model treated with tumor cells genetically modified with 4-1BBL or β-gal, we examined the immune cells in CT26-WT, CT26-βgal, and CT26-4-1BBL tumor bearing mice 21 days after tumor cell administration. The CD8+ T cells population in mice treated with tumor cells genetically modified with 4-1BBL was significantly increased on day 21 compared to that of tumor cells genetically modified with β-gal in the spleen and tumor tissue. The CD4+ T cell population was not different between the two mice groups. The Foxp3+CD25high CD4 T cell population decreased on day 21 in tumor tissues, but the decrease was not significant. We also found that CD8 T cells had pivotal roles in inhibiting tumor growth by treating mice with ant-CD4 and CD8 antibodies. These results suggest that tumor cells genetically modified with 4-1BBL could inhibit tumor growth by affecting on CD8 T lymphocytes.
Keywords
CD8 T cells; Regulatory T cells; Tumor growth inhibition; 4-1BBL;
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