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http://dx.doi.org/10.15616/BSL.2021.27.2.59

Relationship between AdeABC Efflux Pump Genes and Carbapenem in Multidrug-resistant Acinetobacter baumannii  

Ju, Yeongdon (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
Kim, Yoo-Jeong (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
Chang, Chulhun L. (Department of Laboratory Medicine, Pusan National University Yangsan Hospital)
Choi, Go-Eun (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
Hyun, Kyung-Yae (Department of Clinical Laboratory Science, Dong-Eui University)
Publication Information
Biomedical Science Letters / v.27, no.2, 2021 , pp. 59-68 More about this Journal
Abstract
Multidrug-resistant strain of Acinetobacter baumannii (MDRAB) is an emerging pathogen in health care facilities, preventing MDRAB is a public health concern. We conducted this experiment on a clinical isolate of A. baumannii with two main goals: the role of the efflux pump system in the stress provision of carbapenem and the response to the transcription level of the efflux pump gene. A total of 34 strains of A. baumannii was isolated from the Yangsan Hospital of Pusan National University. First, when we compared and observed the expression of the efflux pump gene and antibacterial resistance to carbapenem, a strong correlation was observed between carbapenem resistance and overexpression of adeB (P=0.0056). Second, a correlation between the efflux pump and concentration gradient and tolerance to carbapenem stress at the AdeABC efflux pump genes transcription level was confirmed. Our results revealed that the expression of the AdeABC efflux pump is an important resistance determinant in obtaining antibiotic resistance of the carbapenem group in A. baumannii.
Keywords
Acinetobacter baumannii; Carbapenem; Drug-resistance; Efflux pumps; AdeABC; adeB;
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