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http://dx.doi.org/10.15616/BSL.2021.27.1.19

Effects of Omega-3 Fatty Acid on Endotoxin-induced Acute Lung Injury in Rabbits  

Jang, Eun-A (Department of Anesthesiology and Pain Medicine Chonnam National University Medical School & Hospital)
Son, Sung-Kuk (Department of Anesthesiology and Pain Medicine Chonnam National University Medical School & Hospital)
Kang, Jeong-Hyeon (Department of Anesthesiology and Pain Medicine Chonnam National University Medical School & Hospital)
Lee, Seongheon (Department of Anesthesiology and Pain Medicine Chonnam National University Medical School & Hospital)
Kwak, Sang-Hyun (Department of Anesthesiology and Pain Medicine Chonnam National University Medical School & Hospital)
Publication Information
Biomedical Science Letters / v.27, no.1, 2021 , pp. 19-27 More about this Journal
Abstract
This study was undertaken to clarify the effects of omega-3 fatty acid on endotoxin-induced acute lung injury. Rabbits were randomly assigned to one of four groups. Each group received intravenous infusion of saline only, saline and Escherichia coli endotoxin, omegaven infuison (0.5 mL/kg/hr) and endotoxin, lipoven (0.5 mL/kg/hr) and endotoxin respectively. Infusion of saline was started 0.5 hr before the infusion of saline or endotoxin, and omegaven and lipoven were started 2 hours after endotoxin infusion for 4 hours. The lungs of rabbits were ventilated with 40% oxygen. Mean blood pressure, heart rate, arterial oxygen tension (PaO2), and peripheral blood leukocyte were recorded. The wet/dry (W/D) weight ratio of lung and lung injury score were measured, and analysis of bronchoalveolar lavage fluid (BALF) was done. Endotoxin decreased PaO2, and peripheral blood leukocyte and platelet count. And it increased W/D ratio of lung, lung injury score and leukocyte count, percentage of PMN cells, concentration of IL-8 in BALF. Omegaven attenuated all these changes except for peripheral blood leukocyte counts. Omegaven attenuated endotoxin-induced acute lung injury in rabbits mainly by inhibiting neutrophil and IL-8 responses, which may play a central role in endotoxin-related lung injury.
Keywords
Acute lung injury; Endotoxins; Systemic inflammatory response syndrome; Omegaven;
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