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http://dx.doi.org/10.15616/BSL.2018.24.3.175

Transcription Factor PU.1 Inhibits Aspergillus fumigatus Infection via Surfactant Protein-D  

Kim, Sung-Su (Department of Biomedical Laboratory Science, Daejeon University)
Publication Information
Biomedical Science Letters / v.24, no.3, 2018 , pp. 175-182 More about this Journal
Abstract
Aspergillosis is a life-threatening disease in individuals with compromised immune systems. Fungal invasion is a highly critical process during host cellular infection. Several papers have reported that transcription factors are responsible for the infection process. To investigate what transcription factors are involved in the process in an effort to inhibit fungal infection into cells, I checked the surfactant protein family and PU.1 transcription factor levels in A549 cells infected with A. fumigatus conidia. PU.1 and surfactant protein-D levels were reduced in cells infected with fungal conidia. I then observed an increase in surfactant protein-D on PU.1-overexpressed cells. Infection of A. fumigatus conidia was decreased in PU.1-overexpressed cells, whereas the suppression of PU.1 did not lead to any changes in cases of A. fumigatus conidia infection. These results indicate that PU.1 inhibits the infection of A. fumigatus conidia via the expression of surfactant protein-D, suggesting that PU.1 is a key transcription factor for protection against A. fumigatus invasion.
Keywords
Aspergillosis; Aspergillus fumigatus; PU.1; Surfactant Protein-D;
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