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Anti-inflammatory Effect of Indirubin-3'-Monoxime-5-Sulphonic Acid on Lipopolysaccharide-stimulated Murine Macrophage  

Park, Gang-Baek (Department of Biomedical Science, Catholic University of Daegu)
Kim, Hyun-Jin (Department of Biomedical Science, Catholic University of Daegu)
Heo, Hye-Seon (Department of Biomedical Science, Catholic University of Daegu)
Park, Geun-Mook (Department of Biomedical Science, Catholic University of Daegu)
Park, Kyung-Woo (Department of Biomedical Science, Catholic University of Daegu)
Kim, Jin-Kyung (Department of Biomedical Science, Catholic University of Daegu)
Publication Information
Biomedical Science Letters / v.17, no.3, 2011 , pp. 225-230 More about this Journal
Abstract
Indirubin is the active ingredient of Danggui Longhui Wan, a mixture of plants that is used in traditional Chinese medicine to treat chronic diseases. In this study we investigated the anti-inflammatory effects of an indirubin derivative, indirubin-3’-monoxime-5-sulphonic acid (I3M-5S, $C_{16}H_{11}N_3O_5S$). We found that I3M-5S inhibits the production of various inflammatory mediators such as nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) as well as inflammatory cytokines, tumor necrosis factor-${\alpha}$ and interleukin-6 in lipopolysaccharide (LPS) stimulated murine macrophage, RAW264.7 cells. In addition, the expression of inducible nitric oxide synthase and cyclooxygenase-2, which are essential enzymes to produce NO and $PGE_2$, respectively, was blocked by I3M-5S treatment in LPS-stimulated RAW264.7 cells. Present data suggest that I3M-5S exhibits potent anti-inflammatory activity in cultured macrophages and merit further study as potential therapeutic agents for inflammatory disorders.
Keywords
Inflammation; Indirubin-5-sulphonic acid; Macrophage; Lipopolysaccaride; RAW264.7 cell;
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