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Preventive Effect of Natural Pigments Against Ultraviolet B-induced Cell Death in HaCat Cells  

Lim, Jae-Chung (Bio-therapy Human Resources Center, Animal Medical Center, Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Bae, Chun-Sik (Bio-therapy Human Resources Center, Animal Medical Center, Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Jeong, Soo-Young (Hanbang Industrial Promotion Institutes)
Boo, Hee-Ock (Department of Biotechnology, College of Nature Science, Chosun University)
Hwang, Seong-Jin (Department of Biology, College of Nature Science, Chonnam National University)
Lim, Seul-Ki (Bio-therapy Human Resources Center, Animal Medical Center, Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Park, Min-Jung (Bio-therapy Human Resources Center, Animal Medical Center, Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Kim, Jong-Chun (Bio-therapy Human Resources Center, Animal Medical Center, Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Kang, Seong-Soo (Bio-therapy Human Resources Center, Animal Medical Center, Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Han, Ho-Jae (Bio-therapy Human Resources Center, Animal Medical Center, Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Park, Soo-Hyun (Bio-therapy Human Resources Center, Animal Medical Center, Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
Publication Information
Biomedical Science Letters / v.17, no.1, 2011 , pp. 55-60 More about this Journal
Abstract
Skin is a physical barrier against diverse injury and damages. Exposure to ultraviolet (UV) radiation causes detrimental skin injuries such as inflammation and cell death. The value of natural pigments could be applied to many usages including cosmetics. This study was conducted to examine the protective effect of natural pigments extracted from mulberry, balsam pear, purple-colored sweet potato, pehmannia root, gardenia fruit, and black rice against UV-induced cell death in HaCaT cells, human keratinocyte cell lines. In the present study, the exposure of 50 mJ/$cm^2$ UV-B for 24 hr induced cell death in HaCaT cells, which was prevented by the pretreatment of extracts of mulberry, balsam pear, purple-colored sweet potato, rehmannia root, gardenia fruit, and black rice. In addition, the exposure of 50 mJ/$cm^2$ UV-B for 24 hr also increased lipid peroxide (LPO) formation, compared to control in HaCaT cells, which was prevented by the pretreatment of extracts of mulberry, balsam pear, purple-colored sweet potato, rehmannia root, gardenia fruit, and black rice. In conclusion, the extracts of mulberry, balsam pear, purple-colored sweet potato, rehmannia root, gardenia fruit, and black rice prevented the UV-B-induced cell apoptosis via the inhibition of oxidative stress in HaCaT cells.
Keywords
Apoptosis; Keratinocytes; Natural pigments; Oxidative stress; Ultraviolet B;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Kim SH, Joo MH, Yoo SH. Structural identification and antioxidant properties of major anthocyanin extracted from Omija (Schizandra chinensis) fruit. J Food Sci. 2009. 74: C134-C140.   DOI   ScienceOn
2 Lee IK, Kim CJ, Song KS, Kim HM, Koshino H, Uramoto M, Yoo ID. Cytotoxic benzyl dihydroflavonols from Cudrania tricuspidata. Phytochemistry. 1996. 41: 213-216.   DOI   ScienceOn
3 Liu HR, Tang XY, Dai DZ, Dai Y. Ethanol extracts of Rehmannia complex (Di Huang) containing no Corni fructus improve early diabetic nephropathy by combining suppression on the ET-ROS axis with modulate hypoglycemic effect in rats. J Ethnopharmacol. 2008. 118: 466-472.   DOI   ScienceOn
4 Masaki H. Role of antioxidants in the skin: anti-aging effects. J Dermatol Sci. 2010. 58: 85-90.   DOI   ScienceOn
5 Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979. 95: 351-358.   DOI   ScienceOn
6 Park YS, Kim SJ, Chang HI. Isolation and anthocyanin from black rice (Heugjinjubyeo) and screening of its antioxidant activities. Kor J Microbiol Biotechnol. 2008. 36: 55-60.
7 Sin HJ. A trend in research and development of natural gardenia pigments. Kor J Biotechnol Bioeng. 2007. 22: 271-277.
8 Solovchenko A, Schmitz-Eiberger M. Significance of skin flavonoids for UV-B-protection in apple fruits. J Exp Bot. 2003. 54: 1977-1984.   DOI
9 Sung YY, Yoon T, Jang JY, Park SJ, Kim HK. Topical application of Rehmannia glutinosa extract inhibits mite allergen-induced atopic dermatitis in NC/Nga mice. J Ethnopharmacol. 2011. (in press)
10 Teoh SL, Latiff AA, Das S. The effect of topical extract of Momordica charantia (bitter gourd) on wound healing in nondiabetic rats and in rats with diabetes induced by streptozotocin. Clin Exp Dermatol. 2009. 34: 815-822.   DOI   ScienceOn
11 Wlaschek M, Tantcheva-Poor I, Naderi L, Ma W, Schneider LA, Razi-Wolf Z, Schuller J, Scharffetter-Kochanek K. Solar UV irradiation and dermal photoaging. J Photochem Photobiol. B 2001. 63: 41-51.   DOI   ScienceOn
12 Cho EJ, Yokozawa T, Rhyu DY, Kim HY, Shibahara N, Park JC. The inhibitory effects of 12 medicinal plants and their component compounds on lipid peroxidation. Am J Chin Med. 2003. 31: 907-917.   DOI   ScienceOn
13 Cserhati T, Forgacs E, Morais MH, Mota T. Liquid chromatography of natural pigments. Biomed Chromatogr. 2000. 14: 281-286.   DOI   ScienceOn
14 Dinkova-Kostova AT. Phytochemicals as protectors against ultraviolet radiation: versatility of effects and mechanisms. Planta Med. 2008. 74: 1548-1559.   DOI   ScienceOn
15 Hou Z, Qin P, Ren G. Effect of anthocyanin-rich extract from black rice (Oryza sativa L. Japonica) on chronically alcohol induced liver damage in rats. J Agric Food Chem. 2010. 58:3191-3196.   DOI   ScienceOn
16 Hyung SH, Min KJ, Kim YC. Alleviating effect of Gardeniae fructus water extract on inflammation and skin-barrier damage. The Journal of Cosmetological Science. 2010. 6: 63-72.
17 Isoir M, Buard V, Gasser P, Voisin P, Lati E, Benderitter M. Human keratinocyte radiosensitivity is linked to redox modulation. J Dermatol Sci. 2006. 41: 55-65.   DOI   ScienceOn
18 Kanakis CD, Tarantilis PA, Polissiou MG, Diamantoglou S, Tajmir-Riahi HA. An overview of DNA and RNA bindings to antioxidant flavonoids. Cell Biochem Biophys. 2007. 49: 29-36.   DOI   ScienceOn
19 An RB, Sohn DH, Kim YC. Hepatoprotective compounds of the roots of Cudrania tricuspidata on tacrine-induced cytotoxicity in Hep G2 cells. Biol Pharm Bull. 2006. 29: 838-840.   DOI   ScienceOn
20 Assefa Z, Laethem AV, Garmyn M, Agostinis P. Ultraviolet radiation-induced apoptosis in keratinocytes: on the role of cytosolic factors. Biochim Biophys Acta. 2005. 1755: 90-106.
21 Burke KE. Photoaging: the role of oxidative stress. G Ital Dermatol Venereol. 2010. 145: 445-459.
22 Chang SH, Jung EJ, Lim DG, Oyungerel B, Lim KI, Her E, Choi WS, Jun MH, Choi KD, Han DJ, Kim SC. Anti-inflammatory action of Cudrania tricuspidata on spleen cell and T lymphocyte proliferation. J Pharm Pharmacol 2008. 60: 1221-1216.   DOI   ScienceOn
23 Chaturvedi P. Bitter melon protects against lipid peroxidation caused by immobilization stress in albino rats. Int J Vitam Nutr Res. 2009. 79: 48-56.   DOI
24 Chen CJ, Huang CY, Huang JK, Lin CY, Lin CT. Cloning, Expression, and Purification of a Functional Glutathione Reductase from Sweet Potato (Ipomoea batatas [L.] Lam): Kinetic Studies and Characterization. J Agric Food Chem. 2011. (in press)