Browse > Article

Effects of Nitric Oxide on the Neuronal Activity of Rat Cerebellar Purkinje Neurons  

Jang, Su-Joong (Department of Physiology, Chonnam National University Medical School)
Jeong, Han-Soong (Department of Physiology, Chonnam National University Medical School)
Park, Jong-Seong (Department of Physiology, Chonnam National University Medical School)
Publication Information
Biomedical Science Letters / v.16, no.4, 2010 , pp. 259-264 More about this Journal
Abstract
This study was designed to investigate the effects of nitric oxide on the neuronal activity of rat cerebellar Purkinje cells. Sprague-Dawley rats aged 14 to 16 days were decapitated under ether anesthesia. After treatment with pronase and thermolysin, the dissociated Purkinje cells were transferred into a chamber on an inverted microscope. Spontaneous action potentials and potassium current were recorded by standard patch-clamp techniques under current and voltage-clamp modes respectively. 15 Purkinje cells revealed excitatory responses to $20\;{\mu}M$ of sodium nitroprusside (SNP) and 4 neurons (20%) did not respond to SNP. Whole potassium currents of Purkinje cells were decreased by SNP (n=10). Whole potassium currents of Purkinje cells were also decreased by L-arginine, substrate of nitric oxide (n=10). These experimental results suggest that nitric oxide increases the neuronal activity of Purkinje cells by altering the resting membrane potential and after hyperpolarization.
Keywords
Nitric oxide; Purkinje cell; L-arginine; Neuronal activity;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kaplan DR, Miller FD. Neurotrophin signal transduction in the nervous system. Curr Opin Neurobiol. 2000. 10: 381-391.   DOI   ScienceOn
2 Lee JH, Price RH, Williams FG, Mayer B, Beitz AJ. Nitrix oxide synthase is found in spinothalamic neurons and in neuronal processes that appase spinal neurons that express Fos induced by noxious stimualtion. Brain Res. 1993. 608: 324-333.   DOI   ScienceOn
3 Meller ST, Dykstra C, Gebhart GF. Production of endogenous nitric oxide and activation of soluble guanylate cyclase are required for N-methyl-D-aspartate-produced facilitation of the nociceptive tail-flick reflex. Eur J Pharmacol. 1992. 214: 93-96.   DOI   ScienceOn
4 Sah P, Faber ES. Channels underlying neuronal calcium-activated potassium currents. Prog Neurobiol. 2002. 66: 345-353.   DOI   ScienceOn
5 Sansom AJ, Darlington CL, Smith PF. Intraventricular injection of an NMDA antagonists disrupts vestibular compensation. Neuropharmacol. 1990. 29: 83-84.   DOI   ScienceOn
6 Schilling K, Schmindt HHHW, Baader SL. Nitric oxide synthase expression reveals compartments of cerebellar granule cells and suggests a rele ofr mossy fibers in their development. Neurosscience 1994. 59: 893-903.   DOI   ScienceOn
7 Aoki M, Miyata H, Mizuta K, Ito Y. Evidence for the involvement of NMDA receptors in vestibular compensation. J Vet Res. 1996.6: 315-317.
8 Bon C, Bohm GA, Doble A, Stutzmann JM, Blanchard JC. A role for nitric oxide in long term potentiation. Eur J Neurosci. 1994.4: 420-424.
9 Smith PF, Darlington CL. The NMDA antagonist MK801 and CPP disrupt compensation for unilateral labyrinthectomy in the guinea pig. Neurosci Lett. 1988.94: 309-313.   DOI   ScienceOn
10 Nagao S, Kitamura T, Nakamura N, Hiramatsu T, Yamada J. Differences of the primate flocculus and ventral paraflocculus in the mossy and climbing fiber input organization. J Comp Neurol. 1997. 382: 480-498.   DOI   ScienceOn
11 Kitahara T, Saika T, Takeda N, Kubo T, Kiyama H. Changes in Fos and jun expression after labyrinthectomy in the rat brainstem. Acta Otolaryngol Suppl. 1995. 520: 401-404.
12 Johnston AR, MacLeod NK, Dutia MS. Ion conductances contributing to spike repolarization and afterpotential in rat medial vestibular nucleus neurons. J Physiol. 1994.481: 61-77
13 Kitahara T, Takeda N, Saika T, Kubo T, Kiyama H. Effects of MK801 on Fos expression in the rat brainstem after unilateral labyrinthectomy. Brain Res. 1995.700: 182-190.   DOI
14 Matsumoto T, Pollock JS, Nakane M, Forstermann U. Developmental changes of cytosolic and particulate nitric oxide synthase in rat brain. Dev Brain Res. 1994. 73: 199-203.
15 McCabe BF, Ryu JH, Sekitani T. Further experiments on vestibula rcompensation. Laryngoscope 1972. 82: 381-396.   DOI   ScienceOn
16 Holscher C, Rose SP. Inhibiting synthesis of the putative retrograde messenger nitric oxide results in amnesia in a passive avoidance task in the chick. Brain Res. 1993. 619: 189-194.   DOI
17 Ito M. The cerebellum and neural control. 1985. pp.13-20. Raven Press, NY. USA.
18 Kay AR, Wong RK. Isolation of neurons suitable for patchclamping from adult mammalian central nervous systems. J Neurosci Methods. 1986. 16: 227-238.   DOI   ScienceOn
19 Kim MS, Jin BK, Chun SW, Lee MY, Lee SH, Kim JH, Park BR. Role of vestibulocerebellar NMDA for behavioral recovery following unialteral labyrinthectomy in rats. Neurosci Lett. 1997. 222: 171-174.   DOI   ScienceOn
20 Darlington CL, Flohr H, Smith PF. Molecular mechanisms of brainstem plasticity. The vestibular compensation model. Mol-Neurobiol. 1991. 5: 355-368.   DOI   ScienceOn
21 Dawson TM, Dawson VL, Snyder SH. A novel neuronal messenger molecule in the brain: the free radical, nitric oxide. Ann Neurol. 1992.32: 297-311.   DOI   ScienceOn
22 Furman JM, Balaban CD, Pollack IF. Vestibular compensation in a patient with a cerebellar infarction. Neurology 1997. 48: 916-920.   DOI   ScienceOn
23 Garthwaite J. Glutamate, nitric oxide and cell-cell signaling in the nervous system. TINS. 1991. 14: 60-67.
24 Bredt DS, Snyder SS. Nitric oxide, a novel neuronal messenger. Neuron 1992. 8: 3-11   DOI   ScienceOn
25 Brune B, Lapetina EG. Activation of a cytosolic ADP-ribosyl transferase by nitric oxide-gnerating agents. J Biol Chem. 1989. 264: 8455-8458.
26 Bult H, Boeckxstaens GE, Pelckmans PA, Hordaens, Van Maercke YM, Herman AG. Nitric oxide as an inhibitory non-adrenergic non-cholinergic neurotransmitter. Nature 1990. 345: 346-347.   DOI   ScienceOn
27 Bredt DS, Hwang PM, Snyder SH. Localization of nitric oxide synthase, indicating a neuronal role for nitric oxide. Nature 1990. 347: 768-770.   DOI   ScienceOn