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http://dx.doi.org/10.15324/kjcls.2022.54.4.273

Antimicrobial Effects of 5-Aminolevulinic Acid Mediated Photodynamic Therapy against Pathogenic Bacteria  

Pil Seung, Kwon (Department of Clinical Laboratory Science, Wonkwang Health Science University)
Publication Information
Korean Journal of Clinical Laboratory Science / v.54, no.4, 2022 , pp. 273-278 More about this Journal
Abstract
This study evaluates the improved effect of photodynamic therapy (PDT) by subjecting pathogenic bacteria to a combination of 630 nm light-emitting diode (LED) and 5-aminolevulinic acid (ALA). Bacterial suspensions of 1.5×104 cells/mL were diluted and exposed to ALA concentrations of 10, 5, 2.5, 1.25, and 0.625 mg/mL, incubated for 30 minutes, followed by irradiation with 630 nm LED (18 J/cm2 ). The non-irradiated P. aeruginosa group and the group administered only LED light averaged 415 and 245 colonies, respectively. Conversely, the PDT group showed an average of 109, 225, 297, and 285 colonies at concentrations of 10, 5, 2.5, and 1.25 mg/mL of ALA. Evaluating the effect on E. faecalis revealed an average of 8,750 and 8,000 colonies in the group that did not receive the control photosensitizer and the group exposed to light alone, respectively. However, an average of 0, 2350, 4825, and 7475 colonies at concentrations of 5, 2.5, 1.25, and 0.625 mg/mL ALA were determined for the PDT groups. In conclusion, better inhibitory effects were observed for E. faecalis than for P. aeruginosa. Moreover, our results validate the possibility of improved PDT efficacy using a combination of ALA and 630 nm LED.
Keywords
5-aminolevulinic acid; Enterococcus faecalis; Photodynamic therapy; Pseudomonas aeruginosa;
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