Browse > Article
http://dx.doi.org/10.15324/kjcls.2020.52.4.408

Antioxidative Effect of Aster yomena (Kitm.) Extract on C6 Glioma Cell Line Damaged by AlCl3, Dementia Inducer  

Seo, Young-Mi (Department of Nursing, Wonkwang Health Science University)
Publication Information
Korean Journal of Clinical Laboratory Science / v.52, no.4, 2020 , pp. 408-416 More about this Journal
Abstract
This study examined the neuronal cytotoxicity of aluminum chloride (AlCl3), a dementia inducer, and the protective effects of Aster yomena (Kitam.)(AY) extract on AlCl3-induced cytotoxicity in cultured C6 glioma cells. The antioxidative effects, such as the inhibitory ability of xanthine oxidase (XO) and superoxide anion-radical (SAR) scavenging ability, on cell viability were examined. AlCl3 decreased the cell viability significantly in a dose-dependent manner, and the XTT50 value was 130.0 μM in these cultures. The cytotoxicity of AlCl3 was determined to be mid-toxic according to the Borenfreund and Puerner' toxic criteria. Quercetin (QU), an antioxidant, increased the cell viability reduced by AlCl3-induced cytotoxicity. The protective effect of the AY extract on AlCl3-induced cytotoxicity was analyzed. The AY extract increased the cell viability remarkably compared to the AlCl3-treated group and showed the inhibitory ability of XO and SAR-scavenging ability. The cytotoxicity of AlCl3 was correlated with oxidative stress, and the AY extract effectively prevented AlCl3-induced cytotoxicity through its antioxidative effects. In conclusion, natural resources, such as the AY extract, may be a putative agent for improving the cytotoxicity of heavy metallic compounds correlated with oxidative stress, such as AlCl3, a morbid agent.
Keywords
$AlCl_3$ metallic compound; Antioxidative effect; Neurotoxicity;
Citations & Related Records
Times Cited By KSCI : 10  (Citation Analysis)
연도 인용수 순위
1 Ellis JM. Cholinesterase inhibitors in the treatment of dementia. JAOA. 2005;105:145-158.
2 Kourie JI. Mechanisms of amyloid β protein-induced modification in ion transport systems: implications for neurodegenerative disease. Cell Mol Neurobiol. 2001;21:173-213. https://doi.org/10.1023/a:1010932603406.   DOI
3 Bryan-Sisneros AA, Fraser SP, Suh YH, Djamgoz MB. Toxic effect of the β-amyloid precursor protein C-terminus fragment and Na+/Ca2+ gradients. Neuroreport. 2000;11:3357-3360. https://doi.org/10.1097/00001756-200010200-00019.   DOI
4 Anon. Water poisoning in cornwall. Lancet. 1988:ii:465.
5 Verstraeten SV, Amio L, Oteiza PI. Aluminum and lead: molecular mechanisms of brain toxicity. Arch Toxicol. 2008;82:789-802. https://doi.org/10.1007/s00204-008-0345-3   DOI
6 Endoh M, Maiese K, Wagner J. Expression of the inducible from of nitric oxide synthase by reactive astrocytes after transient global ischemia. Brain Res. 1994;651:92-100. https://doi.org/10.1016/0006-8993(94)90683-1   DOI
7 Oh YH, Park ST. Protective effect of Prunella vulgaris L. var lilacina Nakai extract on cultured NIH3T3 fibroblasts damaged by mutagenic mercury-induced toxicity. J People Plants Environ. 2015;18:41-46. https://doi.org/10.11628/ksppe.2015.18.1.041   DOI
8 Kim SJ, YU YW, Lee JK. Cytotoxicity and protective effect of Portulaca oleracea L. extract on cultured neuroglioma cells damaged by aluminum of dementia-induced agent. J Kor Soc Plants Environ. 2013;16:251-256.   DOI
9 Ma J, Luo XD, Protiva P, Yang H, Ma C, Basile MJ, et al. Bioactive novel polyphenols from the fruit of Manilkara zapota (Sapodilla). J Nat Prod. 2003;66:983-986. https://doi.org/10.1021/np020576x   DOI
10 Yun J, Woo ER, Lee DG. Isoquercitrin, isolated from Aster yomena triggers ROS-mediated apoptosis in Candida albicans. J Funct Foods. 2016;22:347-357. https://doi.org/10.1016/j.jff.2016.01.041   DOI
11 Lee JY, Park JY, Kim HD, Lee SE, Lee JH, Lee YJ, et al. Anti-oxidant and anti-adipocyte differentiation of Aster glehni and Aster yomena. J Nutr Health. 2019;52:250-257. https://doi.org/10.4163/jnh.2019.52.3.250   DOI
12 Lavid N, Schwartz A, Yarden O, Tel-Or E. The involvement of polyphenols and peroxidase activities in heavy-metal accumulation by epidermal glands of the waterlily (Nymphaeaceae). Planta. 2001;212:323-331. https://www.jstor.org/stable/23386118   DOI
13 Meydani M. Effect of functional food ingredients: vitamin E modulation of cardiovascular diseases and immune status in the elderly. Am J Clin Nutr. 2000;71(6 Suppl):1665-1668. https://doi.org/10.1093/ajcn/71.6.1665S   DOI
14 Sim JH, Lee HS, Lee SY, Park DE, Oh KH, Hwang KA, et al. Anti-asthmatic activities of an ethanol extract of Aster yomena in an ovalbumin-induced murine asthma model. J Med Food. 2013;17:606-611. https://doi.org/10.1089/jmf.2013.2939   DOI
15 Choi JH, Kim DW, Park SE, Choi BS, Sapkota K, Kim S, et al. Novel thrombolytic protease from edible and medicinal plant Aster yomena (Kitam.) Honda with anticoagulant activity: purification and partial characterization. J Biosci Bioeng. 2014;118: 372-377. https://doi.org/10.1016/j.jbiosc.2014.03.004   DOI
16 Borenfreund E, Puerner JA. A simple quantitative procedure using monolayer culture for cytotoxicity assay (HTD/NR-90). J Tiss Cult Meth. 1985;9:7-9.   DOI
17 AOAC. Official methods of analysis. 18th ed. Washington DC: Association of Official Analytical Chemists; 2005. Vol 45, p21-22.
18 Nieva Moreno MI, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol. 2000;71:109-114. https://doi.org/10.1016/S0378-8741(99)00189-0   DOI
19 Stirpe F, Corte ED. The regulation of rat liver xanthine oxidase conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O). J Biol Chem. 1969;244:3855-3861.   DOI
20 Jung JY, Jung IJ, Jekal SJ. The protective effect of Lonicerae flos extract on cultured C6 glioma cells damaged by aluminum of dementia. Korean J Clin Lab Sci. 2017;49:271-278. https://doi.org/10.15324/kjcls.2017.49.3.271   DOI
21 Deloncle R, Guillard. Mechanisms of Alzheimer's disease: arguments for a neurotransmitter-aluminium complex implication. Neurochem Res. 1990;15:1239-1245. https://doi.org/10.1007/BF01208586   DOI
22 Wisniewski HM, Struman JA, Shek JW. Aluminum chloride induced neurofibrillary changes in the developing rabbit: a chronic animal model. Ann Neurol. 1980;8:479-490. https://doi.org/10.1002/ana.410080505   DOI
23 Chung JH, Rim TS, Seo YM. Protective effect of Albizzia julibrissin leaf extract on the cytotoxicity induced by cupric acetate metallic mordant. J Environ Health Sci. 2019;45:520-528. https://doi.org/10.5668/JEHS.2019.45.5.520   DOI
24 Lee SH, Jung IJ, Jang HS. The antioxidative effect of Eclipta prostrata L. extract on cultured NIH3T3 fibroblasts injured by manganese-induced cytotoxicity. Biomed Sci Lett. 2018;24:357-364. https://doi.org/10.15616/BSL.2018.24.4.357   DOI
25 Hwang KA, Hwang YJ, Song J. Aster yomena extract ameliorates pro-inflammatory immune response by suppressing NF-κB activation in RAW 264.7 cells. J Chin Med Assoc. 2018;81:102-110. https://doi.org/10.1016/j.jcma.2017.06.017   DOI
26 Bae JS, Kim TH. Acetylcholinesterase inhibitory and antioxidant properties of Aster yomena extract. Kor J Herbol. 2009;24:121-126.
27 Han MH, Jeong JS, Jeong JW, Choi SH, Kim SO, Hong SH, et al. Ethanol extracts of Aster yomena (Kitam.) Honda inhibit adipogenesis through the activation of the AMPK signaling pathway in 3T3-L1 preadipocytes. Drug Discov Ther. 2017;11:281-287. https://doi.org/10.5582/ddt.2017.01046   DOI
28 Lee YJ, Jang YN, Han YM, Kim HM, Jeong JM, Son MJ, et al. Skin barrier protective effects of Aster glehni extract in human keratinocytes HaCaT cells treated with sodium dodecyl sulfate (SDS detergent) and 2,4-dinitrochlorobenzene (DNCB, dermatitis inducer, sensitizer). Seoul: KFN International Symposium and Annual Meeting; 2016. p408-409.
29 Lee WJ, Woo ER, Lee DG. Effect of apigenin isolated from Aster yomena against Candida albicans: apigenin-triggered apoptotic pathway regulated by mitochondrial calcium signaling. J Ethnopharmacol. 2019;231:19-28. https://doi.org/10.1016/j.jep.2018.11.005   DOI
30 Kim SO, Jeong JS, Choi YH. Antioxidant and anti-inflammatory effects of ethanol extract of Aster yomena in RAW 264.7 macrophages. J Life Sci. 2019;29:977-985. https://doi.org/10.5352/JLS.2019.29.9.977   DOI
31 Abbott NJ, Ronnback L, Hansson E. Astrocyte-endothelial interactions at the blood-brain barrier. Nat Rev Neurosci. 2006;7:41-53. https://doi.org/10.1038/nrn1824.   DOI
32 Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Meth. 1983;65:55-63. https://doi.org/10.1016/0022-1759(83)90303-4   DOI