Browse > Article
http://dx.doi.org/10.15324/kjcls.2020.52.4.372

Evaluation of the Sterilization Effect of a Plasma Generator with a Flexible Electrode Structure on Staphylococcus aureus and Pseudomonas aeruginosa  

Park, Chul (Department of Biomedical Laboratory Science, Gwangju Health University)
Lee, Hyeok Jae (Department of Biomedical Laboratory Science, Gwangju Health University)
Publication Information
Korean Journal of Clinical Laboratory Science / v.52, no.4, 2020 , pp. 372-380 More about this Journal
Abstract
In this study, the sterilization ability of S. aureus and P. aeruginosa was evaluated using a plasma generator with a flexible electrode structure. Both strains were prepared at a concentration of 1.5×106 CFU/mL and inoculated and spread evenly on two medium plates. The medium were kept at a distance of 3 cm and 9 cm from the plasma generator and were plasma discharged from 30 sec to 10 minutes. The growth of colonies on the media, were subsequently compared with the control group. The mean colonies of S. aureus formed at a 3 cm distance were 9.2×102 (log value 2.96) CFU/mL for the 5 min discharge period and 8.0×10 (1.90) CFU/mL for the 10 min discharge period. When the medium was exposed for 5 min and 10 min at a 9 cm distance, the mean colonies of S. aureus formed were 2.16×103 (3.33) and 2.4×102 (2.38) CFU/mL, respectively. The medium containing P. aeruginosa kept at a 3 cm distance and exposed to 3, 5, 10-minute discharge, did not form any colonies. When kept at a 9 cm distance for 3 minutes, 6.0×102 (2.78) CFU/mL mean colonies were formed but no colonies were formed at exposure periods of 5 and 10 minutes. This enhanced sterilization effect was confirmed in experiments of S. aureus and P. aeruginosa using TiO2.
Keywords
Flexible electrode; P. aeruginosa; S. aureus; Sterilization;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Vautier M, Guillard C, Herrmann JM. Photocatalytic degradation of dyes in water: case study of indigo and of indigo carmine. J Catal. 2001;201:46-59. https://doi.org/10.1006/jcat.2001.3232   DOI
2 Yoon YH, Nam SH, Joo JC, Ahn HS. Photocatalytic disinfection of indoor suspended microorganisms (Escherichia coli and Bacillus subtilis spore) with ultraviolet light. Journal of the Korea Academia-Industrial cooperation Society. 2014;15:1204-1210. https://dx.doi.org/10.5762/KAIS.2014.15.2.1204   DOI
3 Gaunt LF, Beggs CB, Georghiou GE. Bactericidal action of the reactive species produced by gas-discharge nonthermal plasma at atmospheric pressure: a review. IEEE Transactions on Plasma Science. 2006;34:1257-1269. https://doi.org/10.1109/TPS.2006.878381   DOI
4 von Woedtke TH, Reuter S, Masur K, Weltmann KD. Plasma for medicine. Phys Rep. 2013;530:291-320. https://doi.org/10.1016/j.physrep.2013.05.005   DOI
5 Fridman G, Friedman G, Gutsol A, Shekhter AB, Vasilets VN, Fridman A. Applied plasma medicine. Plasma Process Polym. 2008;15:503-533. https://doi.org/10.1002/ppap.200700154   DOI
6 Fridman G, Peddinghaus M, Ayan H, Fridman A, Balasubramanian M, Gutsol A, et al. Blood coagulation and living tissue sterilization by floating-electrode dielectric barrier discharge in air. Plasma Chem Plasma Process. 2006;26:425-442. https://doi.org/10.1009/PLASMA.2006.1707305   DOI
7 Daeschlein G, Woedtke TV, Kindel E, Brandenburg R, Weltmann KD, Junger M. Antibacterial activity of an atmospheric pressure plasma jet against relevant wound pathogens in vitro on a simulated wound environment. Plasma Process Polym. 2010;7:224-230. https://doi.org/10.1002/ppap.200900059   DOI
8 Fridman G, Shereshevsky A, Jost MM, Brooks AD, Fridman A, Gutsol A, et al. Floating electrode dielectric barrier discharge plasma in air promoting apoptotic behavior in melanoma skin cancer cell lines. Plasma Chem Plasma Process. 2007;27:163-176. https://doi.org/10.1007/s11090-007-9048-4   DOI
9 Steelman VM. Ethylene oxide. The importance of aeration. AORN J. 1992;55:773-775.   DOI
10 Laroussi M, Lu X. Room-temperature atmospheric pressure plasma plume for biomedical applications. Appl Phys Lett. 2005;87:113902. https://doi.org/10.1063/1.2045549   DOI
11 Kim TY, Na JH, Min BJ, Cho SY. A study on sterilization of Pseudomonas sp. using titanium dioxide photocatalyst. J Adv Eng and Tech. 2011;4:129-133. https://doi.org/10.5762/KAIS.2014.15.2.1204   DOI
12 Laroussi M, Leipold F. Evaluation of the roles of reactive species, heat and UV radiation in the inactivation of bacterial cells by air plasmas at atmospheric pressure. Int. J Mass Spectrom. 2004; 233:81-86. https://doi.org/10.1016/j.ijms.2003.11.016   DOI
13 Moisan M, Barbeau J, Crevier MC, Pelletier J, Philip N, Saoudi B. Plasma sterilization methods and mechanisms. Pure Appl Chem. 2002;74:349-358. https://doi.org/10.1351/pac200274030349   DOI
14 Lee HJ, Song HJ, Song MJ. A study on the effect of microbial sterilization using plasma generator with a flexible electrodes sturcture. J Korean Inst Electr Electron Mater Eng. 2020;33:71-78. https://doi.org/10.4313/JKEM.2020.33.1.71   DOI
15 Son HH, Lee WG. Discharge properties of torch-type atmospheric pressure plasma and its local disinfection of microorganism. Korean Chem Eng Res. 2011;49:835-839. https://doi.org/10.9713/KCER.2011.49.6.835   DOI
16 Bogaerts A, Neyts E, Gijbels R, Joost van der Mullen. Gas discharge plasmas and their application. Spectrochim Acta B. 2002;57:609-658. https://doi.org/10.1016/S0584-8547(01)00406-2   DOI
17 Heinlin J, Morfill G, Landthaler M, Stolz W, Isbary G, Zimmermann JL, et al. Plasma medicine: possible application in dermatology. J Dtsch Dermatol Ges. 2010;8:968-976. https://doi.org/10.1111/j.1610-0387.2010.07495.x   DOI
18 Takenaka K, Okumura Y, Setsuhara Y. Plasma interactions with organic materials in liquid through plasma/liquid interface. Jpn J Appl Phys. 2013;52:1-5. https://doi.org/10.7567/JJAP.52.11NE04   DOI
19 Du CM, Shi TH, Sun YW, Zhuang XF. Decolorization of acid orange 7 solution by gas-liquid gliding arc discharge plasma, J Hazard Mater. 2008;154:1192-1197. https://doi.org/10.1016/j.jhazmat.2007.11.032   DOI
20 Son HH, Lee WG. Treatment of Ar/O2 atmospheric pressure plasma for sterilization. Appl Chem Eng. 2011;22:261-265.
21 Jo JO, Lee HW, Mok YS. Sterilization of scoria powder by corona discharge plasma. Appl Chem Eng. 2014;25:386-391. https://doi.org/10.14478/ace.2014.1046   DOI
22 Kwon YH, Park HM, Song HG, Park WZ. Ozone production characteristics of the DBD discharge the electrode shape at the same electrode surface area. J Korean Inst Illum Electr Install Eng. 2016;30:71-77. https://doi.org/10.5207/JIEIE.2016.30.2.071   DOI
23 Falkenstein Z. Application of dielectric barrier discharges. Proceedings of the 12th International Conference on high-power particle beams. 1998:117-120. https://doi.org/10.1109/beams.1998.822399   DOI
24 Ryu YH, Uhm HS, Park GS, Choi EH. Sterilization of neurospora crassa by noncontacted low temperature atmospheric pressure surface discharged plasma with dielectric barrier structure. J Vac Sci. 2013;22:55-65. https://dx.doi.org/10.5757/JKVS.2013.22.2.55   DOI
25 Lee SJ, Song YS, Park YR, Ryu SM, Jeon HW, Eom SH. Sterilization of food borne pathogenic bacteria by atmospheric pressure dielectric barrier discharge plasma. J Food Hyg Saf. 2017;32: 222-227. https://doi.org/10.13103/jfhs.2017.32.3.222   DOI
26 Kim JE, Kim IH, Min SC. Microbial decontamination of vegetables and spices using cold plasma treatments. Korean J food Sci Technol. 2013;45:735-741. https://doi.org/10.9721/KJFST.2013.45.6.735   DOI
27 Kim KY, Paik NW, Kim YH, Yoo KH. Bactericidal efficacy of non-thermal DBD plasma on Staphylococcus aureus and Escherichia coli. J Korean Soc Occup Environ Hyg. 2018;28:61-79. https://doi.org/10.15269/JKSOEH.2018.28.1.61   DOI
28 Athanasekou CP, Moustakas NG, Morales-Torres S, PastranaMartinez LM, Figueiredo JL, Faria JL, et al. Ceramic photo-catalytic membranes for water filtration under UV and visible light. Appl Catal B: Environ. 2015;178:12-19. https://doi.org/10.1016/j.apcatb.2014.11.021   DOI
29 Ryu YH, Uhm HS, Park GS, Choi EH. Sterilization of Neurospora crassa by noncontacted low temperature atmospheric pressure surface discharged plasma with dielectric barrier structure. J Korean Vac Soc. 2013;22:55-65. https://doi.org/10.5757/JKVS.2013.22.2.55   DOI