Browse > Article
http://dx.doi.org/10.15324/kjcls.2020.52.4.335

Comparison of Quantitative Relationship between Real-Time PCR and Acid Fast Bacilli Staining for Diagnosis of Pulmonary Tuberculosis  

Jung, Taewon (Department of Laboratory Medicine, Samsung Medical Center)
Kim, Sang-Ha (Department of Laboratory Medicine, Konyang University Hospital)
Kim, Sunghyun (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
Choi, Jae-Sun (Department of Biomedical Laboratory Science, Far East University)
Kim, Young-Kwon (Department of Health Sciences, The Graduate School of Konyang University)
Publication Information
Korean Journal of Clinical Laboratory Science / v.52, no.4, 2020 , pp. 335-341 More about this Journal
Abstract
This study investigates the association of the AFB stain with the cycle threshold (Ct) value of the Cobas TaqMan MTB test (CTM test, Roche Diagnostics, Basel, Switzerland), and it establishes the base data for semi-quantitative identification of M. tuberculosis by the Ct value. CTM test were simultaneously conducted on 8,389 specimens submitted to the Samsung Medical Center from January 2015 to December 2015, and the results were analyzed and compared retrospectively investigates the association of the AFB stain with the Ct value of the CTM test, and it establishes the base data for semi-quantitative identification of M. tuberculosis by the Ct value. The Ct values for 135 positive specimens of the CTM were inversely correlated with the AFB stain (rs=-0.545, P<0.01). When the Ct value of the CTM test and the time to positivity (TTP) of the mycobacteria cultures were verified based on the AFB stain, they were found to have a positive correlation (rs=0.136, P<0.01). The negative correlation between the CTM test and the AFB stain grade was demonstrated. The clinical significance was verified by applying these criteria to the clinical results. The semi-quantitative criteria of this study can be used to facilitate the rapid isolation of patients with active tuberculosis and infection control in the hospital.
Keywords
Cycle threshold value; Mycobacterium tuberculosis; Real-time PCR; Smear positivity grade;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Behr MA, Warren SA, Salamon H, Hopewell PC, Ponce de Leon A, Daley CL, et al. Transmission of Mycobacterium tuberculosis from patients smear-negative for acid-fast bacilli. Lancet. 1999;353:444-449. https://doi.org/10.1016/s0140-6736(98)03406-0   DOI
2 Lee MR, Chung KP, Wang HC, Lin CB, Y CJ, Lee JJ, et al. Evaluation of the Cobas TaqMan MTB real-time PCR assay for direct detection of Mycobacterium tuberculosis in respiratory specimens. J Clin Microbiol. 2013;62;1160-1164. https://doi.org/10.1099/jmm.0.052043-0   DOI
3 Joint committee for the revision of Korean guidelines for tuberculosis. Korean guidelines for tuberculosis. 3rd ed. Cheongju: KCDC; 2017. p1-232.
4 WHO. Global tuberculosis report 2018 [Internet]. Geneva: WHO; 2020 [cited 2020 Sep 27]. Available from: https://apps.who.int/iris/handle/10665/274453
5 Dye C, Williams BG. The population dynamics and control of tuberculosis. Science. 2010;328:856-861. https://doi.org/10.1126/science.1185449   DOI
6 Frieden TR, editor. Toman's tuberculosis: case detection, treatment and monitoring, 2nd ed [Internet]. Geneva: WHO; 2020 [cited by 2020 Sep 27] Available from: https://tbrieder.org/publications/books_english/toman_2.pdf
7 Brandli O. The clinical presentation of tuberculosis. Respiration. 1998;65:97-105. https://doi.org/10.1159/000029238   DOI
8 Lee H, Park KG, Lee G, Park J, Park YG, Park YJ. Assessment of the quantitative ability of AdvanSure TB/NTM real-time PCR in respiratory specimens by comparison with phenotypic methods. Ann Lab Med. 2014;34:51-55. https://doi.org/10.3343/alm.2014.34.1.51   DOI
9 Armand S, Vanhuls P, Delcroix G, Courcol R, Lemaitre N. Comparison of the Xpert MTB/RIF test with an IS6110-TaqMan real-time PCR assay for direct detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens. J Clin Microbiol. 2011;49:1772-1776. https://doi.org/10.1128/JCM.01335-13   DOI
10 KCDC. Annual report on the notified tuberculosis in Korea 2018 [Internet]. Cheongju: KCDC; 2020 [cited by 2020 Sep 27]. Available from: https://www.cdc.go.kr/CDC/cms/cmsFileSeDownload.jsp?fid=31&cid
11 Blakemore R, Nabeta P, Davidow AL, Vadwai V, Tahirli R, Munsamy V, et al. A multisite assessment of the quantitative capabilities of the Xpert MTB/RIF assay. Am J Respir Crit Care Med. 2011;184:1076-1084. https://doi.org/10.3343/alm.2014.34.1.51   DOI
12 Theron G, Pinto L, Peter J, Mishra HK, Mishra HK, van Zyl-Smit R, et al. The use of an automated quantitative polymerase chain reaction (Xpert MTB/RIF) to predict the sputum smear status of tuberculosis patients. Clin Infect Dis. 2012;54:384-388. https://doi.org/10.1093/cid/cir824   DOI
13 Koh WJ, Yu CM, Suh GY, Chung MP, Kim H, Kwon OJ, et al. Pulmonary TB and NTM lung disease: comparison of characteristics in patients with AFB smear-positive sputum. Int J Tuberc Lung Dis. 2006;10:1001-1007.
14 Huh HJ, Koh WJ, Song DJ, Ki CS, Lee NY. Evaluation of the Cobas TaqMan MTB test for the detection of Mycobacterium tuberculosis complex according to acid-fast-bacillus smear grades in respiratory specimens. J Clin Microbiol. 2015;53:696-698. https://doi.org/10.1128/JCM.02630-14   DOI
15 Marlowe EM, Novak-Weekley SM, Cumpio J, Sharp SE, Momeny MA, Babst A, et al. Evaluation of the Cepheid Xpert MTB/RIF assay for direct detection of Mycobacterium tuberculosis complex in respiratory specimens. J Clin Microbiol. 2011;49:1621-1623. https://doi.org/10.1128/JCM.02214-10   DOI
16 Long R, Ellis E. Introducing the sixth edition of the Canadian Tuberculosis Standards. Can J Infect Dis Med Microbiol. 2007;18: 283-284. https://doi.org/10.1155/2007/628347   DOI
17 Grant T. How should laboratories measure mycobacterial load? S Afr Resp J. 2015;21:54. https://doi.org/10.7196/10.2015.v21i3.54   DOI
18 Brodie D, Schluger NW. The diagnosis of tuberculosis. Clin Chest Med. 2005;26:247-271. https://doi.org/10.1016/j.ccm.2005.02.012   DOI
19 Han YM, Kim HS, Kim CH, Kang HJ, Lee KM. Analysis of patients with positive acid-fast bacilli culture and negative T-SPOT.TB results. Korean J Lab Med. 2010;30:414-419. https://doi.org/10.3343/kjlm.2010.30.4.414   DOI
20 Laraque F, Griggs A, Slopen M, Munsiff SS. Performance of nucleic acid amplification tests for diagnosis of tuberculosis in a large urban setting. Clin Infect Dis. 2009;49:46-54. https://doi.org/10.1086/599037   DOI
21 Bloemberg GV, Voit A, Ritter C, Deggim V, Bottger EC. Evaluation of Cobas TaqMan MTB for direct detection of the Mycobacterium tuberculosis complex in comparison with Cobas Amplicor MTB. J Clin Microbiol. 2013;51:2112-2117. https://doi.org/10.1128/JCM.00142-13   DOI
22 Gillespie SH, Sabiiti W, Oravcova K. Mycobacterial load assay. Methods Mol Biol. 2017;1616:89-105. https://doi.org/10.1007/978-1-4939-7037-7_5   DOI
23 CAP. 2017 nucleic acid amplification of Mycobacterium tuberculosis. Survey results report. Northfield: CAP; 2017. p5-6.