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http://dx.doi.org/10.15324/kjcls.2019.51.3.370

A Study on the Protective Effect of Antioxidants on Damage Induced by Liver Ischemia/Repefusion in a Rat Model  

Ahn, Yong Ho (Department of Clinical Laboratory Science, Dongnam Health University)
Seok, Pu Reum (Department of Biomedical Laboratory Science, Eulji University)
Oh, Su Jin (Department of Biomedical Laboratory Science, Eulji University)
Choi, Jin Woo (Department of Biomedical Laboratory Science, Eulji University)
Shin, Jae-Ho (Department of Biomedical Laboratory Science, Eulji University)
Publication Information
Korean Journal of Clinical Laboratory Science / v.51, no.3, 2019 , pp. 370-378 More about this Journal
Abstract
The hepatic ischemic model has recently been widely used for the epidemiological study of ischemic reperfusion injury. This study was carried out to investigate the protective effect of vanillin, which is known to have antioxidant and anti-inflammatory effects, against hepatic and renal injury using an ischemia-reperfusion rat model, and we also investigated the mechanism related to vanillins' protective effect. The test material was administered at a concentration of 100 mg/kg for 3 days, followed by ligation of the liver for 60 minutes to induce ischemia reperfusion. As control groups, there was a negative control, sham control and ischemia-reperfusion-only ischemia reperfusion control, and the controls groups were compared with the drug administration group. In the vanillin group, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were significantly inhibited compared with the AST and ALT activities of the ischemia-reperfusion group, and histopathological examination showed significant reduction of both inflammation and necrosis. The malondialdehyde (MDA) and superoxide dismutase (SOD) levels were significantly different from the ischemia-reperfusion group. In conclusion, vanillin showed a hepatocyte protective action by alleviating the cellular inflammation and cell necrosis caused by hepatic ischemia-reperfusion, and vanillin mitigated inflammatory changes in the kidney glomeruli and distal tubules. The protective effect is considered to be caused by vanillin's antioxidant function. Further studies such as on cell death and possibly vanillin's same effect on damaged tissue will be necessary for clinical applications such as organ transplantation.
Keywords
Ischemia-reperfusion; Liver; Kidney; Rat; Vanillin;
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1 Champion HR, Jones RT, Trump BF. A clinicopathologic study of hepatic dysfunction following shock. Surg Gynecol Obstet. 1976;142:657-663.
2 Kanufman RP, Anner H, Kobzik L, Valeri CR, Shepro D, Hechman HB. Vasodilator prostaglandins prevent renal damage after ischemia. Ann Surg. 1987;205:195-198.   DOI
3 Da Silveira M, Yoshida WB. Trimetazidine and Nacetylcysteine in attenuating hind-limb ischemia and reperfusion injuries: Experimental study in rats. Int Angiol. 2009;28:412-417.
4 Lee HT, Park SW, Kim M, D'Agati VD. Acute kidney injury after hepatic ischemia and reperfusion injury in mice. Lab Invest. 2009;89:196-208.   DOI
5 Stephensen KR, Steinberg SM, Hughes KS, Vetto JT, Sugarbaker PH, Chang AE. Perioperative blood transfusions are associated with decreased time to recurrence and decreased survival after resection of colorectal liver metastasis. Ann Surg. 1988;208:679-687.   DOI
6 Matsumata T, Kanematsu T, Shirabe K, Sonoda T, Furuta T, Sugimachi K. Decreased morbidity and mortality rates in surgical patients with hepatocellular carcinoma. Br J Surg. 1990;77:677-680.   DOI
7 Taniguchi H, Takahashi T. Analysis of 210 elective hepatic resections. Hepatogastroenterology. 1997;44:1624-1631.
8 Fujimoto J, Okamoto E, Yamanaka N, Tanaka T, Tanaka W. Adverse effect of perioperative blood transfusions on survivial after hepatic resection for epatocellular ciacinoma. Hepatogastroenterology. 1997;44:1390-1396.
9 Demetrios V, Nikolaos A, Georgia K, Sofia S, Loukas K, Dimitrios D, et al. Deferoxamine attenuates lipid peroxidation, blocks Interleukin-6 production, ameliorates sepsis inflammatory response syndrome, and confers renoprotection after acute hepatic ischemia in pigs. Artif Organs. 2012;36:400-408.   DOI
10 Daemen MA, Vreis BD, Buurman WA. Apoptosis and inflammation in renal reperfusion injury. Transplantation. 2002;73:1693-1700.   DOI
11 Seifi B, Kadkhodaee M, Delavari F, Mikaeili S, Shams S, Ostad SN. Pretreatment with pentoxifylline and N-acetylcysteine in liver ischemia reperfusion-induced renal injury. Ren Fail. 2012;34:610-615.   DOI
12 Korthuis RJ, Granger DN. Reactive oxygen metabolites, neutrophils, and the pathogenesis of ischemic-tissue/reperfusion. Clin Cardiol. 1993;16(4 Suppl 1):19-26.   DOI
13 Cheng WY, Wu SL, Hsiang CY, Li CC, Lai TY, Lo HY, et al. Relationship between San-Huang-Xie-Xin-Tang and its herbal components on the gene expression profiles in HepG2 cells. Am J Chin Med. 2008;36:783-797.   DOI
14 Tsung A, Hiroyuki T, Nakao A, Fink MP, Lotze MT, Yang H, et al. The nuclear factor HMGB1 mediates hepatic injury after murine liver ischemiareperfusion. J Exp Me. 2005;201:1135-1143.   DOI
15 Brand K. Aerobic glycolysis by proliferating cells: protection against oxidative stress at the expense of energy yield. J Bioenerg Biomembr. 1997;29:355-364.   DOI
16 Lirdprapamongkol K, Sakurai H, Kawasaki N, Choo MK, Saitoh Y, Aozuka Y, et al. Vanillin suppresses in vitro invasion and in vivo metastasis of mouse breast cancer cells. Eur J Pharm Sci. 2005:25:57-65.   DOI
17 Liang JA, Wu SL, Lo HY, Hsiang CY, and Ho TY. Vanillin inhibits matrix metalloproteinase-9 expression through down-regulation of nuclear factor-B signaling pathway in human hepatocellular carcinoma cells. Mol Pharmacol. 2009:75:151-157.   DOI
18 Durant. S. and Kaman. P. Vanillins- a novel family of DNA-PK inhinitors. Nucleiic Acids Res. 2003;31:5501-5512.   DOI
19 Fitzgerald DJ, Stratford M, Gasson MJ, Ueckert J, Bos A, Narbad A. Mode of antimicrobial action of vanillin against Escherichia coli, Lactobacillus plantarum and Listeria innocua. J Appl Microbiol. 2004:97:104-113.   DOI
20 Santosh Kumar SS, Priyadarsini KI, Sainis KB. Inhibition of peroxynitrite-mediated reactions by vanillin. J Agric Food Chem. 2004:52:139-145.   DOI
21 Nankivell BJ, Chapman JR. Chronic allograft nephropathy: current concepts and future directions. Transplantation. 2006;81:643-654.   DOI
22 Roumen RM, Hendriks T, van der Ven-Jongekrig J, Nieuwenhuijzen GA, Sauerwein RW, van der Meer JW, et al. Cytokine patterns in patients after major vascular surgery, hemorrhagic shock, and severe blunt trauma, Relation with subsequent adult respiratory distress syndrome and multiple organ failure. Ann Surg. 1993;218:769-776.   DOI
23 Klebanoff SJ, Vadas MA, Harian JM, Sparks LH, Gamble JR, Agosti JM, et al. Stimulation of neutrophils by tumor necrosis factor. J Immunol. 1986;136:4220-4225.
24 Gomez-Cambronero LG, Sabater L, Pereda J, Cassinello N, Camps B, Vina J, et al. Role of cytokines and oxidative stress in the pathophysiology of acute pancreatitis: therapeutical implications. Current Drug Targets Inflamm Allergy. 2002;1:393-403.   DOI
25 Zingarelli B, Sheehan M, Wong HR. Nuclear factor-kappa B as a therapeutic target in critical care medicine. Critical Care Medicine. 2003;31(1 Suppl):105-111.   DOI
26 Sasaki H, Matsuno T, Tanaka N, Orita K. Activation of apoptosis during the reperfusion phase after rat liver ischemia. Transplant Proc. 1996;28:1908-1909.
27 Horii Y, Muraguchi A, Iwano M, Matsuda T, HirayamamT, Yamada H, et al. Involvement of IL-6 in measangial proliferative glomerulonephritis. J Immunol. 1989:143:3949-3955.
28 Wald R, Waikar SS, Liangos O, Pereira BI, Chertow GM, Laber BL. Acute renal failure after endovascular vs open repair of abdominal aortic aneurysm. J Vasc Surg. 2006;43:460-466.   DOI
29 Matas AJ, Payne WD, Simmons RL, Buselmeier TJ, Kjellstrand CM. Acute renal failure following blunt civilian trauma. Ann Surg. 1977;185:301-306.   DOI
30 Dame JB, Juul SE. The distribution of receptors for the pro-inflammatory cytokines interleukin (IL)-6 and IL-8 in the developing human fetus. Early Hum Dev. 2000;58:25-39.   DOI
31 Taniguchi Y, Yorioka N, Kumagai J, Katsutani M, Kuratsune M, Amimoto D, et al. Interleukin-6 localization and the prognosis of Ig A nephropathy. Nephron. 1999:81:94-98.   DOI
32 Akagi K, Hirose M, Hoshiya T, Mizoguchi Y, Ito N, Shirai T. Modulating effects of ellagic acid, vanillin and quercetin in a rat medium term multi-organ carcinogenesis model. Cancer Lett. 1995;94:113-121.   DOI
33 Ruiz-Ortega M, Ruperez M, Lorenzo O, Esteban V, Blanco J, Mezzano S, et al. Angioyrndion II regulates the synthesis of proinflammatory cytokines and chemokines in the kidney. Kid Int suppul. 2002:82:12-22.
34 Ahn EK, Jeon HJ, Lim EJ, Jung HJ, Park EH. Anti-inflammatoryand anti-angiogenic activities of Gastrodia elata Blume. J Ethnophamacol. 2007;110:476-482.   DOI
35 Lirdprapamongkol K, Sakurai H, Kawasaki N, Choo MK, Saitoh Y, Aozuka Y, et al. Vanillin suppresses in vitro invasion and in vivo metastasis of mouse breast cancer cells. Eur J Pharm Sci. 2005;25:57-65.   DOI
36 Kaynar K, Gul S, Ersoz S, Ozdemir F, Ulusoy H, Ulusoy S. Amikacin-induced nephropathy: Is there any protective way? Ren Fail. 2007;29:23-27.   DOI
37 Homis E, Ribeiro-Alves MA, Lopes de Faria JB, Dias EP. Interleukin-6 stimulates tubular regeneration in rats with glycerol-induced acute renal failure. Nephron. 2002;92:192-199.   DOI
38 Nam JH, Park HS. Effect of quality and quantity of dietary fat on status of tocopherol and lipid peroxidation of plasma tissue in rats. Korean J Nutr. 1993;26:566-577.
39 Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. 3rd ed. New York, USA: Oxford University Press; 1999.
40 Kang YH, Park YK, Ha TY, Moon KD. Effects of pine needle extract on enzyme activities of serum and liver and liver morphology in rats fed high fat diet. J Korean Soc Food Nutr. 1996;25:374-378.
41 Zaitone S, Hassan N, El-Orabi N, El-Awady S. Pentoxifylline and melatonin in combination with pioglitazone ameliorate experimental non-alcoholic fatty liver disease. Eur J Pharmacol. 2011;662:70-77.   DOI