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http://dx.doi.org/10.15324/kjcls.2019.51.2.245

Effects of 2-methoxy-1,4-naphthoquinone (MQ) on MCP-1 Induced THP-1 Migration  

Kim, Si Hyun (Department of Clinical Laboratory Science, Semyung University)
Park, Bo Bin (Department of Clinical Laboratory Science, Semyung University)
Hong, Sung Eun (Department of Clinical Laboratory Science, Semyung University)
Ryu, Sung Ryul (Department of Clinical Laboratory Science, Semyung University)
Lee, Jang Ho (Department of Clinical Laboratory Science, Semyung University)
Kim, Sa Hyun (Department of Clinical Laboratory Science, Semyung University)
Lee, Pyeongjae (School of Industrial Bio-Pharmaceutical Science, Semyung University)
Cho, Eun-Kyung (Department of Biomedical Laboratory Science, Kyungwoon University)
Moon, Cheol (Department of Clinical Laboratory Science, Semyung University)
Publication Information
Korean Journal of Clinical Laboratory Science / v.51, no.2, 2019 , pp. 245-251 More about this Journal
Abstract
This study examined the effects of 2-methoxy-1,4-naphthoquinone (MQ) on the monocyte chemoattractant protein-1 (MCP-1)-induced migration of monocytes, which is an important phenomenon for the body defense and immune response. MQ is a major component extracted from Impatiens balsamina leaves, which have been used for many years in Asian medicine for the treatment of a range of diseases and pain. The cytotoxicity of MQ began to appear at a concentration of $10{\mu}M$, and approximately 50% cytotoxicity was confirmed at $100{\mu}M$. The MCP-1 induced migration of the THP-1 monocyte cell line increased after MQ treatment in a dose dependent manner and the largest increase was observed at $0.1{\mu}M$. The level of cAMP expression decreased after a treatment with $0.1{\mu}M$ MQ. The phosphorylation of extracellular signal-regulated kinases 1/2 (Erk1/2), a key signaling protein involved in the signaling pathway of C-C motif chemokine receptor 2 (CCR2), a receptor for MCP-1, was increased by the simultaneous treatment of $0.1{\mu}M$ MQ. These results show that MQ increases the MCP-1-induced migration of THP-1, decreases the level of cAMP expression, and increases the level of Erk1/2 phosphorylation.
Keywords
2-methoxy-1,4-naphthoquinone; Migration; Monocyte; Monocyte chemoattractant protein 1;
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