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http://dx.doi.org/10.15324/kjcls.2019.51.1.71

Menadione Induced Apoptosis in MKN45 Cells via Down-regulation of Survivin  

Lee, Min Ho (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
Kim, Jeongyong (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
Cho, Yoonjung (Forensic DNA Division, National Forensic Service)
Kim, Do Hyun (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
Yang, Ji Yeong (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
Kwon, Hye Jin (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
Park, Min (Department of Biomedical Laboratory Science, Daekyeung University)
Woo, Hyun Jun (Department of Clinical Laboratory Science, College of Medical Sciences, Daegu Haany University)
Kim, Sa-Hyun (Department of Clinical Laboratory Science, Semyung University)
Kim, Jong-Bae (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
Publication Information
Korean Journal of Clinical Laboratory Science / v.51, no.1, 2019 , pp. 71-77 More about this Journal
Abstract
Menadione is known as an anti-tumor factor. Many studies have reported the potential anti-cancer role of menadione against a range of cancer cell lines. In this study, the anti-cancer effects of menadione and the underlying molecular signaling involved in apoptosis was investigated in gastric cancer cell lines. The menadione treatment decreased the cell viability of MKN45 gastric cancer cells. The decreased cell viability was attributed to the induction of apoptosis, which was confirmed by the results indicating the activation of caspase-3 and -7 and the cleavage of PARP in Western blotting. The upstream regulatory molecules involved in apoptosis were investigated further and it was discovered that menadione reduced the expression of survivin, an inhibitor of upstream apoptosis proteins. In addition, a transcription factor ${\beta}$-catenin, which is known to regulate survivin expression, was down-regulated by menadione. A previous report showed that menadione inhibited XIAP expression to induce apoptosis and induced G2/M cell cycle arrest in AGS cells. This study elucidated another inhibitory mechanism of menadione against gastric cancer cells in a different cell line. Although further studies will be needed, the inhibitory mechanism demonstrated in this study will help better understand the anti-cancer effects of menadione.
Keywords
Apoptosis; ${\beta}$-catenin; Menadione; MKN45 cells; Survivin;
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