Browse > Article
http://dx.doi.org/10.15324/kjcls.2018.50.4.414

Two-year Quaternary Isolation of Gram-positive Bacilli Using MALDI-TOF MS in Positive Blood Culture of a University Hospital  

Choi, Jin-Un (Department of Laboratory Medicine, Chonnam National University Hospital)
Yu, Young-Bin (Department of Biomedical Laboratory Science, College of Medical Sciences, Konyang University)
Kim, Sang-Ha (Department of Laboratory Medicine, Konyang University Hospital)
Won, Seungho (Department of Mechanical Engineering, Konyang University)
Kim, Young-Kwon (Department of Biomedical Laboratory Science, College of Medical Sciences, Konyang University)
Publication Information
Korean Journal of Clinical Laboratory Science / v.50, no.4, 2018 , pp. 414-421 More about this Journal
Abstract
In this study, Gram-positive bacilli (GPB) were identified by MALDI-TOF MS and analyzed according to the quaternary and microbial strains in the blood culture medium over a two year period at a university hospital. The results were as follows. The overall positive rate of blood culture was 9.97%. In 713 isolated GPB, 410 strains (57.5%) were identified using a microflex MALDI Biotyper. The positive rate of GPB among the blood culture positive bacteria was 8.2%, and the quarterly isolation rate was 9.8% in the third quarter of 2015, 8.7% in the second quarter of 2016, 8.1% in the third quarter of 2016, 8.1% in the first quarter of 2015, 7.9% in the first quarter of 2015, 7.9% in the second quarter of 2015, 6.8% in the first quarter of 2016, and 6.7% in the fourth quarter of 2015. The isolates were Corynebacterium striatum 89 (12.4%), Bacillus cereus 60 (8.4%), Bacillus subtilis 30 (4.2%), Paenibacillus urinalis 29 (4.1%), and Listeria monocytogenes 25 (3.5%). The results of 16S rRNA sequencing of 43 isolates (86.0%) were consistent with those of the other 50 isolates. Five out of the seven unmatched weeks were not identified by MALDI-TOF MS.
Keywords
Blood culture; Gram positive bacilli; Isolation rate; MALDI-TOF MS;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Funke G, von Graevenitz A, Clarridge J3, Berneard KA. Clinical microbiology of coryneform bacteria. Clin Microbiol Rev. 1997;10:125-159.   DOI
2 Adderso EE, Boudreaux JW, Hayden RT. Infections caused by coryneform bacteria in pediatric oncology patients. Pediatr Infect Dis J. 2008;27:136-141. https://doi.org/10.1097/INF.0b013e31814fab12.
3 Barberi SC, Almuzara M, Join-Lambert O, Ramirez MS, Famiglietti A, Vay C. Comparison of the Bruker MALDI-TOF mass spectrometry system and conventional phenotypic methods for identification of Gram-positive rods. PLoS One. 2014:9:e106303. https://doi.org/10.1371/journal.pone.0106303.   DOI
4 Watson RS, Carcillo JA, Linde-Zwirble WT, Clermont G, Lidicker J, Angus DC. The pidemiology of severe sepsis in children in the united stated. Am J Respir Crit Care Med. 2003;167:695-701. https://doi.org/10.1164/rccm.200207-682OC.   DOI
5 Holland RD, Wilkes JG, Rafii F, Sutherland JB, Persons CC, Voorhees KJ, et al. Rapid identification of intact whole bacteria based on spectral patterns using matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 1996;10:1227-1237. https://doi.org/10.1002/(SICI)10970231(19960731)10:10<1227::AIDRCM659>3.0.CO;2-6.   DOI
6 Park KG, Yu YB, Yook KD, Kim SH, Kim SH, Kim YG. An evaluation of the rapid antimicro bial susceptibility test by VITEK MS and VITEK 2 Systems in blood culture. Korean J Clin Lab Sci. 2017;49:279-284. https://doi.org/10.15324/kjcls.   DOI
7 Seng P, Drancourt M, Gouriet F, La Scola B, Fournier PE, Rolain JM, et al. Ongoing evolution in bacteriologyl routine identification of bacteria by matrix-assisted laser desorption ionization itme-offlight mass spectrometry. Clin Infect Dis. 2009;49:543-551. https://doi.org/10.1086/600885.   DOI
8 Bizzini A, Durussel C, Bille J, Greub G, Prod'hom G. Performance of matrix-assisted laser desorption ionizationtime of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory. J Clin Microbiol. 2010;48: 1549-1554. https://doi.org/10.1128/JCM.01794-09.   DOI
9 Stevenson LG, Drake SK, Shea YR, Zelazny AM, Murray PR. Evaluation of matrix-assisted laser desorption ionization-time of Flight mass spectrometry for identification of clinically important yeast species. J Clin Microbiol. 2010;48:3482-3486. https://doi.org/10.1128/JCM.00687-09.   DOI
10 Carbonnelle E, Grohs P, Jacquier H, Day N, Tenza S, Dewailly A, et al. Robustness of two MALDI-TOF mass spectrometry systems for bacterial identification. J Microbiol Methods. 2012;89:133-136. https://di.org/10.1016/j.mimet.2012.03.003.   DOI
11 Bruins MJ, Bloembergen P, Ruijs GJ, Wolfhagen MJ. Identification and susceptibility testing of enterobacteriaceae and pseudomonas aeruginosa by direct inoculation from pos itive BACTEC blood culture bottles into VITEK 2. J Clin Microbiol. 2004;42:7-11. https://doi.org/10.1128/JCM.42.1.7-11.2004.   DOI
12 Dubois D, Grare M, Prere MF, Segonds C, Marty N, Oswald E. Performances of the Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid identification of bacteria in routine clinical microbiology. J Clin Microbiol. 2012;50:2568-2576. https://doi.org/10.1128/JCM.00343-12.   DOI
13 Ahn GY, Jang SJ, Lee SH, Jeong OY, Bidur Prasad Chaulagain BP, Moon DS, et al. Trends of the species and antimicrobial susceptibility of microorganisms isolated from blood cultures of patients. Korean J Clin Microbiol. 2006;9:42-50.
14 Navas M, Pincus DH, Wilkey K, LaSalvia M, Wilson D, Procop GW, et al. Identification of aerobic Gram-positive bacilli by use of Vitek MS. J Clin Microbiol. 2014;52:1274-1277. https://doi.org/10.1128/JCM.03483-13.   DOI
15 Kim NH, Hwang JH, Song KH, Choe PG, Park WB, Kim ES, et al. Changes in antimicrobial susceptibility of blood isolates in a university hospital in South Korea, 1998-2010. Infect Chemother. 2012;44:275-281. https://doi.org/10.3947/ic.2012.44.4.275.   DOI
16 Woo PC, Lau SK, Teng JL, Tse H, Yuen KY. Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. Clin Microbiol Infect. 2008;14:908-934. https://doi.org/10.1111/j.1469-0691.2008.02070.x.   DOI
17 Shin KS, Son YI, Kim TD, Hong SB, Park JS, Kim SH, et al. Secular trends of species and antimicrobial resistance of blood isolates in a tertiary medical center for ten years: 2003-2012. Biomedical Science Letters. 2014;20:2288-7415.
18 Severo CB, Guazzelli LS, Barra MB, Hochhegger B, Severo LC. Multiple pulmonary nodules caused by Corynebacterium striatum in an immunocompetent patient. Rev Inst Med Trop Sao Paulo. 2014;56:89-91. https://doi.org/10.1590/S0036-46652014000100015.   DOI
19 Choi SK, Han MH, Bae CW, Choi YS. A case of paenibacillus-induced sepsis complicated with pneumotocele in a very low birth weight infant. Neonatal Med. 2014;21:69-73. https://doi.org/10.5385/nm.2014.21.1.69.   DOI
20 Renom F, Gomila M, Garau M, Gallegos M.D.C, Guerrero D, Lalucat J, et al. Respira tory infection by Corynebacterium striatum: epidemiological and clinical determinants. New Micorbes New Infect. 2014;2:106-114. https://doi.org/10.1002/nmi2.48.   DOI
21 Levesque S, Dufresne PJ, Soualhine H, Doming M-C, Bekal S, Lefebvre B, et al. A side by side compariton of Bruker Biotyper and VITEK MS Utility of MALDI-TOF MS technolo gy for microorganism identification in a public health reference laboratory. PLoS ONE. 2015;10:e0144878. https://doi.org/10.1371/journal.pone.0144878.   DOI