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http://dx.doi.org/10.35399/ISK.34.1.2

Evidence of Intrusion of a Rare Species, Peristedion liorhynchus, into Korean Waters Based on High-throughput Sequencing of the Mixed Fish Eggs  

Choi, Hae-young (Oceanic Climate & Ecology Research Division, Korea Institute of Ocean Science & Technology)
Chin, Byung-sun (Department of Marine Biotechnology, Anyang University)
Park, Gyung-soo (Department of Marine Biotechnology, Anyang University)
Kim, Sung (Oceanic Climate & Ecology Research Division, Korea Institute of Ocean Science & Technology)
Publication Information
Korean Journal of Ichthyology / v.34, no.1, 2022 , pp. 8-15 More about this Journal
Abstract
The appearance of larvae of a rare species, Peristedion liorhynchus, in Korean waters is suggestive of spawning or adult intrusion. We conducted high-throughput sequencing (HTS) on 31,776 pelagic fish eggs collected from 123 stations off the Korean Peninsula during May to August in 2013, 2014 and 2017. A total of 21,621,874 HTS reads were mapped onto the P. liorhynchus COX1 reference sequence. Three consensus sequences (313 bp) were constructed from the three samples, respectively, off Uljin and Goeje Islands in May and off Ulsan in July. These samples were formed a clade with P. liorhynchus in the maximum likelihood tree of Peristedion. The average genetic distance within the P. liorhynchus clade (0.0054±0.0046) was less than that among clades (0.1475±0.0396). The results indicate that the HTS analysis of mixed fish eggs is useful for monitoring the intrusion of rare species such as P. liorhynchus in Korean waters.
Keywords
COX1; fish eggs; high-throughput sequencing; Peristedion liorhynchus;
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